C6805

Monoclonal Anti-Collagen, Type VII antibody produced in mouse

clone LH7.2, ascites fluid

• Synonym(s): Anti-EBD1, Anti-EBDCT, Anti-EBR1, Anti-NDNC8
• MDL number: MFCD00164570
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: ascites fluid
• antibody product type: primary antibodies
• clone: LH7.2, monoclonal
• contains: 15 mM sodium azide
• species reactivity: human, goat, marmoset, monkey, guinea pig, sheep, pig, bovine
• technique(s): dot blot: suitable; immunocytochemistry: suitable; indirect ELISA: suitable; indirect immunofluorescence: 1:1,000 using human or other mammalian frozen sections; western blot: suitable
• isotype: IgG1
• UniProt accession no.: Q02388
• shipped in: dry ice
• storage temp.: −20°C
• Gene Information: human ... COL7A1(1294)

Description

General description

Collagen is a fibrous protein present in the extracellular framework of all vertebrates. Collagen type VII is the crucial component of anchoring fibrils and mutation in type VII collagen gene (COL7A1) leads to epidermolysis bullosa dystrophica[1]. Monoclonal anti-collagen, type VII antibody can be used to distinguish invasive melanoma from non-invasive melanoma by visualizing the appearance and integrity of epidermal basement membrane. It can also be used in immunoblotting. Mouse anti-collagen type VII antibody reacts specifically with an epitope located on collagenase digested type VII collagen (150 kDa). The product also reacts with the basement membrane zone of stratified squamous epithelia of sheep, pig, bovine, guinea pig, goat and human.

Collagen type VII alpha 1 chain (COL7A1) is majorly expressed in skin, secreted by keratinocytes and fibroblasts.[2][3] The gene is located on human chromosome 3p21.31.[4]

Immunogen

insoluble fractions of neonatal human foreskin epidermal cells

Application

Monoclonal Anti-Collagen, Type VII antibody produced in mouse has been used in immuno-gold labelling.[5]

Monoclonal anti-collagen type VII antibody can be used as a primary antibody in virus titration[6]. It can also be used for the localization of type VII collagen in different immunochemical assays like dot blot, immunocytochemistry and ELISA.

Biochem/physiol Actions

Collagen type VII alpha 1 chain (COL7A1) is required for epithelium-to-stroma anchorage in skin, mucosa and cornea.[2] It is considered as an immediate-early response gene for transforming growth factor β (TGF-β)/SMAD (mothers against decapentaplegic) signaling pathway.[7]

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable