C7048

Monoclonal Anti-CD3 antibody produced in mouse

clone UCHT-1, purified immunoglobulin, buffered aqueous solution

• Synonym(s): Monoclonal Anti-CD3
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: UCHT-1, monoclonal
• form: buffered aqueous solution
• species reactivity: human
• technique(s): flow cytometry: 5 μL using 1 × 10⁶ cells
• isotype: IgG1
• UniProt accession no.: P07766
• shipped in: wet ice
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... CD3E(916)

Description

Specificity

Recognizes the CD3 complex. The epitope recognized by clone no. UCHT-1 is expressed on the ε-chain of the CD3 antigen/T cell receptor complex. 3rd Workshop: code no. 126, 208 and 471
4th Workshop: paper no. T3.2

Immunogen

human thymocytes followed by Sezary T cells.

Application

Monoclonal Anti-CD3 antibody produced in mouse is suitable for flow cytometry at a dilution of 5μL using 1 × 10⁶ cells.

Biochem/physiol Actions

The CD3e cytoplasmic domain of the T cell receptor binds to the inner leaflet of the plasma membrane (PM) and is accessible during early stages of T cell activation.[1] It is specific for T lymphocytes, found in Purkinje cells of the cerebellum and gastric parietal cells of humans and other species. The antibodies commonly used to recognize CD3 are directed against the ε-subunit of the T cell receptor. Cytoplasmic cyCD3 expression can only be achieved during the early phase of NK-cell differentiation.

Target description

The CD3 complex (16/20/29/22/25-28 kDa) consists of 5 polypeptide chains associated with each other and with the T cell receptor.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable