C7113

Anti-Calcitonin Gene-Related Peptide antibody, Mouse monoclonal

clone 4901, purified from hybridoma cell culture

• Synonym(s): Anti-CALC, Anti-CCALCI, Anti-CGRP-I
• MDL number: MFCD00162203
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: 4901, monoclonal
• form: buffered aqueous solution
• species reactivity: rat, human, canine
• packaging: antibody small pack of 25 μL
• concentration: ~2 mg/mL
• technique(s): immunocytochemistry: suitable; indirect ELISA: 0.5 μg/mL using rat α-CGRP; neutralization: suitable; radioimmunoassay: suitable
• isotype: IgG1
• UniProt accession no.: P01258
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... CALCA(796) ; rat ... Calca(24241)

Description

General description

CGRP (Calcitonin gene-related peptide) belongs to calcitonin family and is expressed mainly in the central and peripheral nervous systems. It is a strong endogenous vasodilator. CGRP concentration increases in migraine attacks and hence, it is involved in the pathogenesis of migraine headaches . Monoclonal anti-calcitonin gene-related peptide antibody can be used in immunoneutralization and radioimmunoassay. Mouse anti-calcitonin gene-related peptide antibody reacts specifically with an epitope present within the C-terminal ten amino acids of rat α-CGRP and also has shown cross reactivity with α and β-CGRP of humans and dogs.

Calcitonin gene-related peptide (CGRP) is a vasodilator that belongs to the calcitonin family of peptides. It is produced by motor neurons of peripheral and central nervous system. There are two forms of CGRP, α and β, which exhibit similar functions. The effects of CGRP are mediated by G-protein coupled receptor heterodimer formed by calcitonin-receptor-like receptor (CRLR) and receptor-activity-modifying protein 1 (RAMP1). Several biological effects of CGRP are observed in nervous, endocrine, immune and cardiovascular systems. Multiple studies have implicated CGRP in the pathophysiology of migraine and antagonists to CGRP are being explored as treatment options
Monoclonal Anti-Calcitonin Gene-Related Peptide (CGRP) recognizes rat α-CGRP. The antibody cross-reacts with human and dog α- and β-CGRP.

Immunogen

rat α-CGRP peptide. The epitope recognized by the antibody resides within the C-terminal ten amino acids of rat α-CGRP.

Application

Monoclonal anti-calcitonin gene-related peptide antibody can be used in immunocytochemistry and immunohistochemistry. It can also be used in ELISA.

Anti-Calcitonin Gene-Related Peptide (CGRP) antibody is suitable for ELISA at a working concentration of 0.25- 0.5 μg/ml. The antibody may be used for immunofluorescence at a working dilution of 1:100 in rat nerve fibres and human dorsal root ganglia.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Immunohistochemistry (1 paper)

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable