MilliporeSigma
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C7207

Sigma-Aldrich

Monoclonal Anti-β-Catenin antibody produced in mouse

clone 15B8, ascites fluid

Synonym(s):
Monoclonal Anti-β-Catenin
MDL number:
NACRES:
NA.41

Quality Level

biological source

mouse

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

15B8, monoclonal

mol wt

antigen 94 kDa

species reactivity

human, bovine, chicken, canine

technique(s)

immunocytochemistry: suitable
immunohistochemistry (frozen sections): suitable
indirect immunofluorescence: 1:1000 using cultured MDBK cells
microarray: suitable
western blot: 1:1000 using cultured MDBK cells extract

isotype

IgG1

UniProt accession no.

application(s)

research pathology

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... CTNNB1(1499)

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General description

Monoclonal Anti-β-Catenin (mouse IgG1 isotype) is derived from the 15B8 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. β-catenin belongs to the armadillo family and comprises the N-terminal region with 12 imperfect repeats and a conserved C-terminal region. The locus 3p22.1 in the human chromosome harbors β-catenin gene. β-catenin shares 70% sequence identity to a protein encoded by Drosophila armadillo, a segment polarity gene. Both armadillo and β-catenin share considerable homology with plakoglobin, which has been proposed to be γ-catenin.

Specificity

Recognizes specifically mammalian and avian β-catenin. Does not cross-react with plakoglobin (γ-catenin).

Immunogen

recombinant chicken β-catenin.

Application

Monoclonal Anti-β-Catenin antibody produced in mouse has been used:
  • immunoblotting
  • immunohistochemistry
  • immunolabeling
  • immunofluorescence
  • immunocytochemical staining

Biochem/physiol Actions

β-catenin is a prime component of the wingless (Wnt) pathway and plays a key role in gene expression during development. The homology between β-catenin and armadillo raised the possibility that β-catenin has a developmental signaling role in vertebrates. β-catenin mediates the interaction of the E-cadherin-catenin complex with the epidermal growth factor (EGF) receptor. β-catenin and plakoglobin are substrates for tyrosine phosphorylation following EGF stimulation of cells. β-catenin also associates with the tumor suppressor protein adenomatous polyposis coli (APC). Monoclonal antibody reacting specifically with β-catenin is an essential tool in defining the interactions and distributions of β-catenin and its relationships with other catenins and cadherins in various cells and tissues.

Physical form

The product is provided as ascites fluid with 0.1% sodium azide as a preservative.

Storage and Stability

For extended storage freeze in working aliquots. For continuous use, store at 2-8 °C for up to one month. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Quotes and Ordering

The WNT signaling pathway and its role in human solid tumors
Thorstensen L and Lothe RA
Atlas of Genetics and Cytogenetics in Oncology and Haematology, 7(2), 146-161 (2003)
Swapnalee Sarmah et al.
PloS one, 11(8), e0161205-e0161205 (2016-08-25)
Fetal alcohol spectrum disorder (FASD), birth defects associated with ethanol exposure in utero, includes a wide spectrum of congenital heart defects (CHDs), the most prevalent of which are septal and conotruncal defects. Zebrafish FASD model was used to dissect the
Maryam Yousefi et al.
The Journal of cell biology, 215(3), 401-413 (2016-11-02)
Regeneration of the intestinal epithelium is driven by multiple intestinal stem cell (ISC) types, including an active, radiosensitive Wnt
Matthew T Peterson et al.
Developmental dynamics : an official publication of the American Association of Anatomists, 239(3), 905-913 (2010-01-12)
Hedgehog (Hh) signaling and laminin-111, a basement membrane protein, are required for early muscle development. Hh signaling specifies different populations of muscle fibers and laminin-111 is critical for early muscle morphogenesis. However, additional requirements for Hh signaling and laminin during
Chelsi J Snow et al.
Developmental dynamics : an official publication of the American Association of Anatomists, 237(9), 2542-2553 (2008-08-30)
After somitogenesis, skeletal muscle precursors elongate into muscle fibers that anchor to the somite boundary, which becomes the myotome boundary. Fibronectin (Fn) is a major component of the extracellular matrix in both boundaries. Although Fn is required for somitogenesis, effects

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