C7521

Sigma-Aldrich

Collagen from human placenta

Bornstein and Traub Type IV, powder

Synonym(s):
Collagen Type 4
CAS Number:
EC Number:
MDL number:
NACRES:
NA.54

Quality Level

biological source

human placenta

description

Sigma Type VI

form

powder

application(s)

cell culture | mammalian: suitable

impurities

HIV, hepatitis B and hepatitis C, none detected

solubility

0.5 M acetic acid: 1 mg/mL

suitability

suitable for substrate for collagenase

Featured Industry

Diagnostic Assay Manufacturing

UniProt accession no.

storage temp.

−20°C

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Packaging

10, 50 mg in poly bottle
5 mg in glass bottle

Biochem/physiol Actions

During development, collagen IV is ubiquitously distributed in BMs. During the maturation process, this network gets partially replaced in a remarkably tissue specific manner, defining BM structure and function. Collagen IV has been shown to bind to platelets, hepatocytes, keratinocytes, endothelial, mesangial, pancreatic cells and some tumor cells.

Components

All collagen molecules are composed of three polypeptide chains arranged in a triple helical conformation, with a primary structure that is mostly a repeating motif with glycine in every third position and proline or 4-hydroxyproline frequently preceding the glycine residue. Type IV collagen occurs only in the basement membranes and contains up to six genetically distinct a-chains.

Preparation Note

This is a lyophilized powder that can be reconstituted in sterile .5 M acetic acid, PBS or water at 1 mg/mL. A PBS solution will be stable for at least one year at -20°C. It is prepared by a modification of the pepsin extraction method of Glanville, R.W., et al.

Analysis Note

An SDS polyacrylamide gel electrophoresis test run under reducing conditions consistent with basement membrane collagen yields three major bands.

Other Notes

Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Be wary of confusing Sigma-type designations with recognized collagen classification types.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Marion Jeanne et al.
Circulation, 131(18), 1555-1565 (2015-03-11)
Collagen type IV alpha1 (COL4A1) and alpha2 (COL4A2) form heterotrimers critical for vascular basement membrane stability and function. Patients with COL4A1 or COL4A2 mutations suffer from diverse cerebrovascular diseases, including cerebral microbleeds, porencephaly, and fatal intracerebral hemorrhage (ICH). However, the...
M L Fulcher et al.
American journal of physiology. Lung cellular and molecular physiology, 296(1), L82-L91 (2008-11-04)
Immortalization of human bronchial epithelial (hBE) cells often entails loss of differentiation. Bmi-1 is a protooncogene that maintains stem cells, and its expression creates cell lines that recapitulate normal cell structure and function. We introduced Bmi-1 and the catalytic subunit...
Anuwat Pinyachat et al.
Toxicon : official journal of the International Society on Toxinology, 57(5), 772-780 (2011-02-22)
Molecular cloning and functional characterization of P-III snake venom metalloproteinases (SVMPs) will give us deeper insights in the pathogenesis of viper bites. This may lead to novel therapy for venom-induced local tissue damages, the complication refractory to current antivenom. The...
Emma Lång et al.
Nature communications, 9(1), 3665-3665 (2018-09-12)
Epithelial sheet spreading is a fundamental cellular process that must be coordinated with cell division and differentiation to restore tissue integrity. Here we use consecutive serum deprivation and re-stimulation to reconstruct biphasic collective migration and proliferation in cultured sheets of...
Nguyen Thu Ngan Trinh et al.
American journal of physiology. Lung cellular and molecular physiology, 295(5), L866-L880 (2008-09-02)
Severe lesions of airway epithelia are observed in cystic fibrosis (CF) patients. The regulatory mechanisms of cell migration and proliferation processes, involved in the repair of injured epithelia, then need to be better understood. A model of mechanical wounding of...

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