C7559

Sigma-Aldrich

Chloroform

≥99%, PCR Reagent, contains amylenes as stabilizer

Synonym(s):
Methylidyne trichloride, Trichloromethane
Empirical Formula (Hill Notation):
CHCl3
CAS Number:
Molecular Weight:
119.38
Beilstein/REAXYS Number:
1731042
EC Number:
MDL number:
PubChem Substance ID:

grade

PCR Reagent

Quality Level

vapor density

4.1 (vs air)

vapor pressure

160 mmHg ( 20 °C)

assay

≥99%

form

liquid

contains

amylenes as stabilizer

packaging

vial of 1.5 mL

application(s)

PCR: suitable

color

colorless

refractive index

n20/D 1.445 (lit.)

bp

60.5-61.5 °C (lit.)

mp

−63 °C (lit.)

density

1.492 g/mL at 25 °C (lit.)

foreign activity

DNase, RNase, none detected

SMILES string

ClC(Cl)Cl

InChI

1S/CHCl3/c2-1(3)4/h1H

InChI key

HEDRZPFGACZZDS-UHFFFAOYSA-N

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Related Categories

Packaging

5 vials in serum bottle

Application

Facilitates recovery of the aqueous phase of PCRs which have been overlaid with mineral oil.

Pictograms

Skull and crossbonesHealth hazard

Signal Word

Danger

Target Organs

Central nervous system

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

UN 1888 6.1 / PGIII

WGK Germany

WGK 3

Flash Point(F)

does not flash

Flash Point(C)

does not flash

Accalia Fu et al.
Diabetologia, 58(7), 1513-1522 (2015-04-16)
Precise regulation of insulin secretion by the pancreatic beta cell is essential for the maintenance of glucose homeostasis. Insulin secretory activity is initiated by the stepwise breakdown of ambient glucose to increase cellular ATP via glycolysis and mitochondrial respiration. Knockout...
Kuan Sun et al.
Nature communications, 6, 6013-6013 (2015-01-15)
Solution-processed organic photovoltaic cells (OPVs) hold great promise to enable roll-to-roll printing of environmentally friendly, mechanically flexible and cost-effective photovoltaic devices. Nevertheless, many high-performing systems show best power conversion efficiencies (PCEs) with a thin active layer (thickness is ~100 nm) that...
Shuiquan Tang et al.
Philosophical transactions of the Royal Society of London. Series B, Biological sciences, 368(1616), 20120318-20120318 (2013-03-13)
Two novel reductive dehalogenases (RDases) that are highly similar to each other but catalyse distinct dechlorination reactions were identified from Dehalobacter-containing mixed cultures. These two RDases were partially purified from crude protein extracts of anaerobic dechlorinating enrichment cultures using blue...
Pramod Kumar et al.
Tissue engineering. Part C, Methods, 21(7), 660-670 (2014-12-24)
Tissue engineering by self-assembly uses the cells' secretome as a regeneration template and biological factory of trophic factors. Despite the several advantages that have been witnessed in preclinical and clinical settings, the major obstacle for wide acceptance of this technology...
Mette Bohl et al.
The American journal of clinical nutrition, 101(4), 870-878 (2015-04-04)
Abdominal obesity and exaggerated postprandial lipemia are independent risk factors for cardiovascular disease (CVD) and mortality, and both are affected by dietary behavior. We investigated whether dietary supplementation with whey protein and medium-chain saturated fatty acids (MC-SFAs) improved postprandial lipid...
Protocols
Protocol using antibody mediated hot start polymerase. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.
Read More
Hot Start Taq Polymerase protocol to reduce non-specific amplification, with MgCl2 Optimization
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Protocol using antibody mediated hot start polymerase with a red dye for easy gel loading. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.
Read More

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