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C7559

Sigma-Aldrich

Chloroform

≥99%, PCR Reagent, contains amylenes as stabilizer

Synonym(s):

Methylidyne trichloride, Trichloromethane

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5 VIALS
$131.00

$131.00

Estimated to ship onApril 07, 2025

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5 VIALS
$131.00

About This Item

Empirical Formula (Hill Notation):
CHCl3
CAS Number:
67-66-3
Molecular Weight:
119.38
Beilstein/REAXYS Number:
1731042
EC Number:
200-663-8
MDL number:
MFCD00000826
UNSPSC Code:
12191502
PubChem Substance ID:
24892970
NACRES:
NA.28
grade:
PCR Reagent
assay:
≥99%
technique(s):
PCR: suitable
bp:
60.5-61.5 °C (lit.)
vapor pressure:
160 mmHg ( 20 °C)

$131.00

Estimated to ship onApril 07, 2025

Request a Bulk Order

grade

PCR Reagent

Quality Level

200

vapor density

4.1 (vs air)

vapor pressure

160 mmHg ( 20 °C)

assay

≥99%

form

liquid

contains

amylenes as stabilizer

packaging

vial of 1.5 mL

technique(s)

PCR: suitable

color

colorless

refractive index

n20/D 1.445 (lit.)

bp

60.5-61.5 °C (lit.)

mp

−63 °C (lit.)

density

1.492 g/mL at 25 °C (lit.)

foreign activity

DNase, RNase, none detected

SMILES string

ClC(Cl)Cl

InChI

1S/CHCl3/c2-1(3)4/h1H

InChI key

HEDRZPFGACZZDS-UHFFFAOYSA-N

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General description

Chloroform is used in pharmaceutical preparations as an anesthetic and as an additive. It is now mainly used as an organic solvent.[1] Chloroform is produced by anthropogenic and natural sources. This abundant halocarbon in the atmosphere is found in ground and surface water sources.[2]

Application

Chloroform has been used in phenol-chloroform extraction to find its ability to purify nucleic acids from blood culture fluids.[3] It has also been used in RNA extraction/isolation[2] and purification.[4]

pictograms

Skull and crossbonesHealth hazard
GHS06,GHS08

signalword

Danger

Hazard Classifications

Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 - STOT SE 3

target_organs

Central nervous system, Liver,Kidney

Storage Class

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

wgk_germany

WGK 3

flash_point_f

does not flash

flash_point_c

does not flash

ppe

Eyeshields, Faceshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000262046
0000348479
0000348479
SHBN1700
0000262046

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Kidney
Encyclopedia of Toxicology (2014)
Accalia Fu et al.
Diabetologia, 58(7), 1513-1522 (2015-04-16)
Precise regulation of insulin secretion by the pancreatic beta cell is essential for the maintenance of glucose homeostasis. Insulin secretory activity is initiated by the stepwise breakdown of ambient glucose to increase cellular ATP via glycolysis and mitochondrial respiration. Knockout
Kuan Sun et al.
Nature communications, 6, 6013-6013 (2015-01-15)
Solution-processed organic photovoltaic cells (OPVs) hold great promise to enable roll-to-roll printing of environmentally friendly, mechanically flexible and cost-effective photovoltaic devices. Nevertheless, many high-performing systems show best power conversion efficiencies (PCEs) with a thin active layer (thickness is ~100 nm) that
Shuiquan Tang et al.
Philosophical transactions of the Royal Society of London. Series B, Biological sciences, 368(1616), 20120318-20120318 (2013-03-13)
Two novel reductive dehalogenases (RDases) that are highly similar to each other but catalyse distinct dechlorination reactions were identified from Dehalobacter-containing mixed cultures. These two RDases were partially purified from crude protein extracts of anaerobic dechlorinating enrichment cultures using blue
Pramod Kumar et al.
Tissue engineering. Part C, Methods, 21(7), 660-670 (2014-12-24)
Tissue engineering by self-assembly uses the cells' secretome as a regeneration template and biological factory of trophic factors. Despite the several advantages that have been witnessed in preclinical and clinical settings, the major obstacle for wide acceptance of this technology
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Protocols

Amplification of DNA Using Jumpstart™ REDTaq® DNA Polymerase (D8187)

Protocol using antibody mediated hot start polymerase with a red dye for easy gel loading. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

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