C7594

Casein Blocking Buffer

Name: Casein Blocking Buffer
Brand: SIGMA
Price: 171 USD
Availability: InStock

for Northern and Southern blotting, powder blend

Synonym(s):

blocking buffer

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1 L

$171.00

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About This Item

UNSPSC Code:

12161700

EC Number:

232-555-1

NACRES:

NA.25

Key Documents

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grade

Molecular Biology

form

powder blend

technique(s)

Southern blotting: suitable

pH

6.8-7.2(reconstituted per directions)

solubility

H₂O: soluble, faintly yellow (dissolves in 5-10 minutes.)

storage temp.

room temp

Quality Level

200

Related Categories

Chemistry & Biochemicals

Biochemicals

Biological Buffers

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1 of 4

This Item	B6429	70955	G7663
Sigma-Aldrich C7594 Casein Blocking Buffer	Sigma-Aldrich B6429 Casein Blocking Buffer 10x	Millipore 70955 5% Alkali-soluble Casein	Sigma-Aldrich G7663 Gelatin blocking buffer
technique(s) Southern blotting: suitable	technique(s) Southern blotting: suitable	technique(s) -	technique(s) -
grade for molecular biology	grade for molecular biology	grade -	grade for molecular biology
Quality Level 200	Quality Level 200	Quality Level -	Quality Level 100
form powder blend	form solution	form liquid	form powder blend
solubility H₂O: soluble, faintly yellow (dissolves in 5-10 minutes.)	solubility -	solubility -	solubility -
pH 6.8-7.2(reconstituted per directions)	pH -	pH -	pH -

General description

Casein is a milk-derived substance routinely used in blocking buffers for Western, Southern and Northern Blot procedures. Blocking agents are used to reduce non-specific binding of detection reagents to the membranes.

Application

Suitable as a blocking Buffer for Western, Southern and Northern Blot membranes. Blocked membranes can then be used in either chemiluminescent, colorimetric, or DIG detection assays.

Features and Benefits

Better blocking performance than dried milk or BSA
Compatible with neutral and positively-charged membranes
Compatible with chemiluminescent, colorimetric , and DIG detection assays

Preparation Note

Dissolve entire contents of bottle in 800 mL molecular biology grade water. Stir until well mixed, then add water to final volume of 1L. Store at 2-8°C for up to one week.

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wgk

WGK 1

Storage Class

11 - Combustible Solids

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Certificates of Analysis (COA)

Lot/Batch Number

0000493000

0000397854

0000364067

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Ochratoxin A in some French wines: application of a direct competitive ELISA based on an OTA-HRP conjugate.

Radoi, A., et al.

Analytical Letters, 42, 1187-1187 (2009)

Method for measuring single-molecule adhesion forces and attachment lifetimes of protein-membrane interactions.

Serapion Pyrpassopoulos et al.

Methods in molecular biology (Clifton, N.J.), 1046, 389-403 (2013-07-23)

Many proteins that reside in the cytoplasm bind directly to cell membranes and play roles in signaling, adhesion, metabolism, cell structure, and cell motility. Several of these membrane-binding proteins, especially cytoskeletal proteins, have mechanical functions that result in the transmission

Relative efficiency of porcine and human cytotoxic T-lymphocyte antigen 4 immunoglobulin in inhibiting human CD4+ T-cell responses co-stimulated by porcine and human B7 molecules.

Tadatsura Koshika et al.

Immunology, 134(4), 386-397 (2011-11-03)

α1,3-Galactosyltransferase gene-knockout pigs transgenic for porcine cytotoxic T-lymphocyte antigen 4 immunoglobulin (pCTLA4-Ig) have been produced to reduce T-cell-mediated rejection following xenotransplantation. The level of soluble pCTLA4-Ig in their blood was greatly in excess of the therapeutic level in patients, rendering

Invasion of host cells by JC virus identifies a novel role for caveolae in endosomal sorting of noncaveolar ligands.

W Querbes et al.

Journal of virology, 80(19), 9402-9413 (2006-09-16)

Invasion of glial cells by the human polyomavirus, JC virus (JCV), leads to a rapidly progressing and uniformly fatal demyelinating disease known as progressive multifocal leukoencephalopathy. The endocytic trafficking steps used by JCV to invade cells and initiate infection are

The effects of oligomerization on Saccharomyces cerevisiae Mcm4/6/7 function.

Xiaoli Ma et al.

BMC biochemistry, 11, 37-37 (2010-09-24)

Minichromosome maintenance proteins (Mcm) 2, 3, 4, 5, 6 and 7 are related by sequence and form a variety of complexes that unwind DNA, including Mcm4/6/7. A Mcm4/6/7 trimer forms one half of the Mcm2-7 hexameric ring and can be

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Protocols

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Casein Blocking Buffer Protocol

Protocol for Casein Blocking Buffer in Southern and Western blotting includes troubleshooting for optimizing signal.

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