11 - Combustible Solids
Unoccupied sites on the solid support must be blocked to detect an immobilized antigen. This protocol describes how to use our Casein Blocking Buffer in Southern and Western blotting applications, including options for fluorescein and DIG detection systems.
An introduction to both Northern and Southern blotting, popular methods for the transfer of macromolecules to membranous support. This article also offers a Southern blot protocol and a northern blot protocol.