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C7749

Sigma-Aldrich

Carbonic Anhydrase II bovine

≥90% (SDS-PAGE), recombinant, expressed in E. coli, ≥5,000 units/mg protein, buffered aqueous solution

All Photos(1)
Enzyme Commission number:
4.2.1.1 (BRENDA, IUBMB)
NACRES:
NA.54

recombinant

expressed in E. coli

Quality Level

200

Assay

≥90% (SDS-PAGE)

form

buffered aqueous solution

specific activity

≥5,000 units/mg protein

mol wt

~29 kDa

shipped in

wet ice

storage temp.

−20°C

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Carbonic Anhydrase II bovine ≥90% (SDS-PAGE), recombinant, expressed in E. coli, ≥5,000 units/mg protein, buffered aqueous solution

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Caspase-1, Human, Recombinant, E. coli

assay

≥90% (SDS-PAGE)

assay

-

assay

≥90% (SDS-PAGE)

assay

≥90% (SDS-PAGE)

form

buffered aqueous solution

form

buffered aqueous glycerol solution

form

solution

form

liquid

specific activity

≥5,000 units/mg protein

specific activity

≥5,000 units/mg protein

specific activity

≥1500 units/mg protein

specific activity

≥25,000 units/mg protein, ≥50,000 units/mL

mol wt

~29 kDa

mol wt

~39 kDa

mol wt

192 kDa

mol wt

-

shipped in

wet ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

Application

Carbonic Anhydrase II bovine has been used as a control in native gel electrophoresis and X-ray absorption spectroscopy characterization of Thalassiosira weissflogii carbonic anhydrase 1.
Carbonic anhydrase is used to create carbon dioxide capture systems and to research various purification techniques . Carbonic anhydrase is also used to study acid-base regulation in fish and carbonic anhydrase type II deficiency syndrome . Product C7749, Bovine carbonic anhydrase II (CA II), has been widely used as a model protein in the investigation of the protein folding process.

Biochem/physiol Actions

Carbonic Anhydrase is a zinc-containing enzyme that catalyzes the reversible conversion of carbon dioxide to bicarbonate. One of its main physiological roles is to maintain the acid-base balance in blood and other tissues. Lack of carbonic anhydrase results in carbonic anhydrase type II deficiency syndrome, which is an autosomal recessive disease that causes osteopetrosis, renal tubular acidosis and brain calcifications . Carbonic anhydrases are metalloenzymes since most active sites contain zinc. Carbonic anhydrases are widely distributed in plant and animal tissues where they are involved in diverse physiological processes, such as photosynthesis, pH homeostasis, calcification, and bone resorption. The enzymes can be localized in the cytosol or mitochondria, be membrane bound with extracellular domains, or be secreted. Bovine carbonic anhydrase II (CA II) is a cytosolic, single-chain, approximately 29 kDa enzyme.

Unit Definition

One unit will decrease th pH of a 20 mM tris buffer from pH 8.3 to 6.3 per minute at 0 °C.

Physical form

Supplied as a solution in 20 mM Tris, pH 7.6, with 150 mM NaCl.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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The active site structure of Thalassiosira weissflogii carbonic anhydrase 1
Cox EH, et al.
Biochemistry, 39(40), 12128-12130 (2000)
K M Gilmour et al.
The Journal of experimental biology, 212(Pt 11), 1647-1661 (2009-05-19)
Carbonic anhydrase (CA) is the zinc metalloenzyme that catalyses the reversible reactions of CO(2) with water. CA plays a crucial role in systemic acid-base regulation in fish by providing acid-base equivalents for exchange with the environment. Unlike air-breathing vertebrates, which
Thomas M Bosley et al.
Brain : a journal of neurology, 134(Pt 12), 3502-3515 (2011-11-29)
Carbonic anhydrase type II deficiency syndrome is an uncommon autosomal recessive disease with cardinal features including osteopetrosis, renal tubular acidosis and brain calcifications. We describe the neurological, neuro-ophthalmological and neuroradiological features of 23 individuals (10 males, 13 females; ages at
Joana da Costa Ores et al.
Chemosphere, 88(2), 255-259 (2012-04-20)
This work presents a study of industrially applicable techniques to obtain a biologically supported carbon dioxide capture system, based on the extraction of carbonic anhydrase from bovine blood. Carbonic anhydrase is a metalloenzyme which catalyzes the reversible hydration of carbon
Yuzhu Bian et al.
Artificial cells, nanomedicine, and biotechnology, 41(1), 60-68 (2013-01-26)
Even though erythrocytes transport both oxygen and carbon dioxide, research on blood substitutes has concentrated on the transport of oxygen and its vasoactivity and oxidative effects. Recent study in a hemorrhagic shock animal model shows that the degree of tissue
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