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C7805

Sigma-Aldrich

Monoclonal Anti-Collagen, Type III antibody produced in mouse

clone FH-7A, ascites fluid

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Synonym(s):
Collagen III Antibody - Monoclonal Anti-Collagen, Type III antibody produced in mouse, Collagen Iii Antibody
MDL number:

biological source

mouse

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

FH-7A, monoclonal

mol wt

antigen 70 kDa

contains

15 mM sodium azide

species reactivity

human, rat

technique(s)

dot blot: suitable
immunohistochemistry (frozen sections): 1-4,000 using frozen sections of human skin
indirect ELISA: suitable
western blot: suitable using a denatured-reduced preparation

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... COL3A1(1281)
rat ... Col3a1(84032)

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This Item
SAB4200749C2456C6805
conjugate

unconjugated

conjugate

-

conjugate

unconjugated

conjugate

unconjugated

antibody form

ascites fluid

antibody form

purified from hybridoma cell culture

antibody form

ascites fluid

antibody form

ascites fluid

clone

FH-7A, monoclonal

clone

FH-7A, monoclonal

clone

COL-1, monoclonal

clone

LH7.2, monoclonal

mol wt

antigen 70 kDa

mol wt

~70 kDa

mol wt

-

mol wt

-

species reactivity

human, rat

species reactivity

rat, human

species reactivity

bovine, human, pig, rat, rabbit, deer

species reactivity

human, goat, marmoset, monkey, guinea pig, sheep, pig, bovine

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General description

Collagen is a fibrous protein present in the extracellular framework of all vertebrates. Collagen type III is a 300 kD molecule that belongs to fibrillar collagens family found mainly in skin, blood vessels, liver, placenta, tongue and thymus. This collagen is directly or indirectly involved in several genetic diseases like Ehlers-Danlos type IV disease. Monoclonal anti-collagen type III antibody can be used to determine the interstitial collagen I and III content in total protein homogenates. It is also used to study the immunolocalization of collagen type III in normal and neoplastic tissue. Mouse monoclonal anti-collagen type III antibody reacts specifically with native and denatured collagen type III but not with collagen types I, II, IV, V, VI, and X.
Collagen is a fibrous protein present in the extracellular framework of all vertebrates. Collagen type III is a 300 kD molecule that belongs to fibrillar collagens family found mainly in skin, blood vessels, liver, placenta, tongue and thymus. This collagen is directly or indirectly involved in several genetic diseases like Ehlers-Danlos type IV disease. Mouse monoclonal anti-collagen type III antibody reacts specifically with native and denatured collagen type III but not with collagen types I, II, IV, V, VI, and X. This product has also shown reactivity for rat and human collagen type III.
Monoclonal Anti-Collagen Type III (mouse IgG1 isotype) is derived from the FH-7A hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with human collagen type III.

Specificity

Monoclonal Anti-Collagen Type III reacts specifically with native and denatured collagen type III. It does not recognize collagen types I, II, IV, V, VI, and X.

Immunogen

human collagen type III

Application

Monoclonal Anti-Collagen, Type III antibody produced in mouse has also been used in enzyme linked immunosorbent assay (ELISA) and immunohistochemistry.
Monoclonal anti-collagen type III antibody has been used in immunofluorescence and western blot.

Biochem/physiol Actions

This collagen is directly or indirectly involved in several genetic diseases like Ehlers-Danlos type IV disease. Monoclonal anti-collagen type III antibody can be used to determine the interstitial collagen I and III content in total protein homogenates.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Fluorescently labeled collagen binding proteins allow specific visualization of collagen in tissues and live cell culture
Krahn KN, et al.
Analytical Biochemistry, 350(2), 177-185 (2006)
R Mayne
Clinical biochemistry, 21(2), 111-115 (1988-04-01)
Monoclonal antibodies have recently been developed against all of the major collagen types isolated from both human and other species. These antibodies have several potential advantages over polyclonal antibodies, and a brief survey will be made of the different antibodies
Corinne N Riggin et al.
Journal of orthopaedic research : official publication of the Orthopaedic Research Society, 37(9), 2035-2042 (2019-05-02)
Overuse-induced tendinopathy is highly prevalent in the general population. Percutaneous fenestration, or dry needling, techniques have been increasing in popularity, but despite their current use, there are no controlled laboratory studies to provide fundamental support for this practice. The objective
The use of mesenchymal stem cells in collagen-based scaffolds for tissue-engineered repair of tendons
Butler DL, et al.
Nature Protocols, 5(5), 849-849 (2010)
Advanced glycation end products as a source of artifacts in immunoenzymatic methods
Kuzan A, et al.
Glycoconjugate Journal, 35(1), 95-103 (2018)

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