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C7855

Sigma-Aldrich

Anti-Cdh1 antibody,Mouse monoclonal

clone DCS-266, purified from hybridoma cell culture

MDL number:
NACRES:
NA.41

Quality Level

biological source

mouse

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

DCS-266, monoclonal

form

buffered aqueous solution

mol wt

antigen 50 kDa

species reactivity

human

concentration

~2 mg/mL

application(s)

immunoprecipitation (IP): suitable
microarray: suitable
western blot: 2-4 μg/mL using a HeLa cell nuclear extract

isotype

IgG1

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... CDH1(999)

General description

Monoclonal Anti-Cdh1 (mouse IgG1 isotype) is derived from the DCS-266 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a recombinant human cadherin-1 (Cdh1).
Cdh1 homophilic major cell adhesion molecule in epithelial cells belongs to cadherin superfamily.

Specificity

Monoclonal Anti-Cdh1 reacts specifically with human Cdh1.

Immunogen

recombinant human Cdh1.

Application

Monoclonal Anti-Cdh1 antibody was used for immunoblotting of lysates in a study of Identification of Novel Human Cdt1-binding Proteins. It is suitable for western blotting with 2-4 μg/mL by using a HeLa cell nuclear extract. It is also suitable for immunoprecipitation and microarray.

Biochem/physiol Actions

Monoclonal Anti-Cdh1 reacts specifically with human Cdh1. In eukaryotes, regulation of cell cycle progression depends on the expression of cyclins proteins. Degradation of mitotic cyclins follows ubiquitin-dependent pathways. The anaphase-promoting complex/cyclosome (APC/C) plays a vital role in progression. Initially at anaphase the APC/C requires the activator protein CDC20 to target securin and cyclin B1 for proteasome-dependent degradation, but later it require Cdh1 to maintain its active state till the next S phase. Another cyclin A/cdk2 mediated phosphorylation of Cdh1 may also prevents the activatory assembly of Cdh1 with APC, thus creating an environment permissive for accumulation of APC targets. It mediates Ca2+ dependent homophilic interactions, as the major adhesion receptor in adherens junctions.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4 containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Certificate of Analysis

Certificate of Origin

Suyang Zhang et al.
Nature, 533(7602), 260-264 (2016-04-28)
In eukaryotes, the anaphase-promoting complex (APC/C, also known as the cyclosome) regulates the ubiquitin-dependent proteolysis of specific cell-cycle proteins to coordinate chromosome segregation in mitosis and entry into the G1 phase. The catalytic activity of the APC/C and its ability...
The tumor-suppressor function of E-cadherin.
H Semb et al.
American journal of human genetics, 63(6), 1588-1593 (1998-12-05)
Reinhard Sigl et al.
Journal of cell science, 122(Pt 22), 4208-4217 (2009-10-29)
The anaphase-promoting complex/cyclosome (APC/C) is essential for progression through mitosis. At anaphase onset, the APC/C requires the activator protein CDC20 to target securin and cyclin B1 for proteasome-dependent degradation, but then depends on the CDC20-related protein FZR1 (also known as...
C Lukas et al.
Nature, 401(6755), 815-818 (1999-11-05)
In mammalian somatic-cell cycles, progression through the G1-phase restriction point and initiation of DNA replication are controlled by the ability of the retinoblastoma tumour-suppressor protein (pRb) family to regulate the E2F/DP transcription factors. Continuing transcription of E2F target genes beyond...
Xiaojing Zhang et al.
Oncology letters, 17(3), 2639-2646 (2019-03-12)
Overexpressed in lung cancer 1 (OLC1) is a potential oncogene overexpressed in human lung cancer and in other types of malignant tumor. The elevated expression of OLC1 contributes to tumor genesis and progression. However, the mechanisms regulating the expression of...

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