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C7902

Sigma-Aldrich

Calcium chloride dihydrate

BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99.0%

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Empirical Formula (Hill Notation):
CaCl2 · 2H2O
CAS Number:
Molecular Weight:
147.01
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.71

product line

BioReagent

Assay

≥99.0%

form

powder

technique(s)

cell culture | insect: suitable
cell culture | mammalian: suitable
cell culture | plant: suitable

mp

176 °C (dec.) (lit.)

solubility

H2O: 294 mg/mL, clear, colorless

SMILES string

O.O.Cl[Ca]Cl

InChI

1S/Ca.2ClH.2H2O/h;2*1H;2*1H2/q+2;;;;/p-2

Inchi Key

LLSDKQJKOVVTOJ-UHFFFAOYSA-L

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This Item
C330621097C5080
Calcium chloride dihydrate BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99.0%

Sigma-Aldrich

C7902

Calcium chloride dihydrate

Essential+ Grade
Calcium chloride dihydrate BioUltra, for molecular biology, ≥99.5% (KT)

Sigma-Aldrich

21097

Calcium chloride dihydrate

Premium Grade
Calcium chloride dihydrate BioXtra, ≥99.0%

Sigma-Aldrich

C5080

Calcium chloride dihydrate

Premium Grade
form

powder

form

powder

form

solid

form

powder

mp

176 °C (dec.) (lit.)

mp

176 °C (dec.) (lit.)

mp

176 °C (dec.) (lit.)

mp

176 °C (dec.) (lit.)

solubility

H2O: 294 mg/mL, clear, colorless

solubility

H2O: 294 mg/mL, clear, colorless

solubility

H2O: 1 M at 20 °C, clear, colorless

solubility

H2O: 1 M, clear, colorless

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

product line

BioReagent

product line

-

product line

BioUltra

product line

BioXtra

General description

Calcium chloride (CaCl2) is a commonly used reagent in biochemistry. It plays important roles in many biological processes, including signal transduction, muscle contraction, and maintenance of cell membrane and cell wall stability. CaCl2 is used in the preparation and transformation of competent Escherichia coli (E. coli) and the transfection of eukaryotic cells with either plasmid DNA or high molecular weight genomic DNA. The CaCl2-mediated electroporation of E. coli with the plasmid DNA pBR322 has been studied. CaCl2 is used in the stabilization and two-dimensional crystallization of the NADH-ubiquinone oxidoreductase (complex I) from E. coli. The crystallization of porcine pancreatic elastase in the presence of CaCl2 along with sodium citrate reveals the binding of calcium in the metal-binding site of the protein. The effect of CaCl2 on the total fluorescence in the polymerization of the tubulin-like filamenting temperature-sensitive mutant Z (FtsZ) division protein of E. coli has been studied by a multiwell fluorescent assay.

Application

Calcium chloride dihydrate has been used:
  • in the formulation of media
  • in sperm collection and preparation
  • in cell culture and protoplast preparation

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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The effects of L-ergothioneine and L-ascorbic acid on the in vitro maturation (IVM) and embryonic development (IVC) of sheep oocytes
PANCARCI S M, et al.
Kafkas U?niversitesi veteriner faku?ltesi dergisi, 16(5) (2010)
Hemolymph analysis and evaluation of newly formulated media for culture of shrimp cells (Penaeus stylirostris)
Shimizu C, et al.
In Vitro Cellular & Developmental Biology. Animal, 37(6), 322-329 (2001)
Biomolecule delivery into canola protoplasts by centrifuging cells with microbubbles
He C, et al.
Febs Letters, 587(3), 285-290 (2013)
Marco Wallenborn et al.
PloS one, 15(7), e0234986-e0234986 (2020-07-08)
Glioblastoma is a common, malignant brain tumor whose disease incidence increases with age. Glioblastoma stem-like cells (GSCs) are thought to contribute to cancer therapy resistance and to be responsible for tumor initiation, maintenance, and recurrence. This study utilizes both SNP
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Antimicrobial resistance (AMR) is one of the most significant health threats to society. A growing body of research demonstrates selection for AMR likely occurs at environmental concentrations of antibiotics. However, no standardized experimental approaches for determining selective concentrations of antimicrobials

Articles

Calcium in Cell Culture

Calcium is an ionically stable divalent cation with important beneficial and toxic properties in cell culture. It is a component of a wide range of cell culture media.

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