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C8104

Sigma-Aldrich

Monoclonal Anti-Ceramide antibody produced in mouse

clone MID 15B4, purified immunoglobulin, buffered aqueous solution

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Synonym(s):
Ceramide Antibody, Ceramide Antibody - Monoclonal Anti-Ceramide antibody produced in mouse
MDL number:
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

MID 15B4, monoclonal

form

buffered aqueous solution

technique(s)

immunohistochemistry (frozen sections): suitable
indirect ELISA: 1:10

isotype

IgM

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

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This Item
C2456B2531I2018
biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

ascites fluid

technique(s)

immunohistochemistry (frozen sections): suitable, indirect ELISA: 1:10

technique(s)

dot blot: suitable, immunohistochemistry (frozen sections): 1:2000 using human or other mammalian frozen sections, indirect ELISA: suitable

technique(s)

immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1000 using sections of intestine from mouse or rat treated in vivo with BrdU

technique(s)

dot blot: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000 using human or animal pancreas, radioimmunoassay: suitable

isotype

IgM

isotype

IgG1

isotype

IgG1

isotype

IgG1

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General description

Ceramide is an endogenous lipid component of a novel biochemical pathway termed the sphingomyelin pathway. Ceramide is produced in response to cellular stimulation by hormones, cytokines and antigens. Ceramide synthesis is mediated either by the salvage pathway by the acylation of sphingosine or sphingolipids hydrolysis or by de novo pathway via dihydroceramide formation. Structurally, ceramides have a sphingoid base, a long-chain amino alcohol linked to fatty acid by an amide bond. Ceramide is generated by hydrolysis of sphingomyelin by sphingomyelinase.

Specificity

Mouse monoclonal clone MID 15B4 anti-Ceramide antibody recognizes free and bound ceramides.

Immunogen

ceramide conjugated to bovine serum albumin.

Application

Monoclonal Anti-Ceramide antibody produced in mouse has been used in:
  • immunohistochemistry
  • immunolabeling in electron microscopy
  • enzyme-linked immunosorbent assay (ELISA)
  • immunoblotting
  • immunoprecipitation
  • as a probe to determine the presence and roles of ceramide in sphingomyelin pathway signaling and the regulation of protein phosphorylation.

Biochem/physiol Actions

Ceramide appears to have a role in mediating biological responses in a wide variety of cell types. Ceramide metabolites such as sphingosine and sphingosine-1-phosphate have potent biological activities of their own. They are directly involved in the proliferation and differentiation of skin cells and regulate skin barrier functionality. Ceramide based analogs are potential anti-tumor agents and are regarded as tumor suppressor lipid. Mechanisms for ceramide action involve regulation of protein phosphorylation via stimulation of a serine/threonine protein phosphatase, a proline-directed kinase and possibly other direct and/or indirect targets. Ceramide is emerging as an intracellular messenger than mediates effects on terminal differentiation and cell proliferation as well as apoptosis or cell death and cell-cycle arrest. The interrelationship of ceramide actions with other bioactive lipids and systems represents an area of active research.

Physical form

Solution in phosphate buffered saline containing 0.5 M NaCl, 0.1% bovine serum albumin, and 0.09% sodium azide.

Preparation Note

Purified from ascites fluid by gel filtration using sephacryl S-300 resin.

Analysis Note

The antibody was characterized on ceramide covalently bound to myoglobin.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable


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Ignazio Restivo et al.
International journal of molecular sciences, 24(2) (2023-01-22)
Cell death program of red blood cells (RBCs), called eryptosis, is characterized by activation of caspases and scrambling of membrane phospholipids with externalization of phosphatidylserine (PS). Excessive eryptosis confers a procoagulant phenotype and is implicated in impairment of microcirculation and
Jormay Lim et al.
Communications biology, 3(1), 45-45 (2020-01-29)
Intraflagellar transport (IFT) proteins are essential for cilia assembly and function. IFT protein mutations lead to ciliopathies, which manifest as variable skeletal abnormalities. However, how IFT proteins regulate cell alignment during bone development is unknown. Here, we show that the
Zi-Jun Sun et al.
JCI insight, 7(24) (2022-12-23)
Accumulating evidence suggests the pathogenic role of immunity and metabolism in diabetic kidney disease (DKD). Herein, we aimed to investigate the effect of complement factor B (CFB) on lipid metabolism in the development of DKD. We found that in patients
ELOVL4-mediated production of very long-chain ceramides stabilizes tight junctions and prevents diabetes-induced retinal vascular permeability
Kady NM, et al.
Diabetes, 67(4), 769-781 (2018)
Insulin resistance, ceramide accumulation, and endoplasmic reticulum stress in human chronic alcohol-related liver disease
Longato L, et al.
Oxidative Medicine and Cellular Longevity, 2012 (2012)

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