C8210

Sigma-Aldrich

CHES

BioXtra, ≥99.0% (titration)

Synonym(s):
2-(Cyclohexylamino)ethanesulfonic acid
Empirical Formula (Hill Notation):
C8H17NO3S
CAS Number:
Molecular Weight:
207.29
Beilstein/REAXYS Number:
2967601
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.25
Pricing and availability is not currently available.

product line

BioXtra

assay

≥99.0% (titration)

form

powder

impurities

≤0.0005% Phosphorus (P)
≤0.1% Insoluble matter

ign. residue

≤0.1%

pH

3.0-5.0 (0.5 M in H2O)

useful pH range

8.6 - 10.0

pKa (25 °C)

9.3

solubility

H2O: 0.5 M, clear, colorless

anion traces

chloride (Cl-): ≤0.05%
sulfate (SO42-): ≤0.05%

cation traces

Al: ≤0.0005%
Ca: ≤0.001%
Cu: ≤0.0005%
Fe: ≤0.0005%
K: ≤0.005%
Mg: ≤0.0005%
NH4+: ≤0.05%
Na: ≤0.01%
Pb: ≤0.001%
Zn: ≤0.0005%

absorption

≤0.05 at 280 in H2O at 0.5 M
≤0.08 at 260 in H2O at 0.5 M

Featured Industry

Diagnostic Assay Manufacturing

SMILES string

OS(=O)(=O)CCNC1CCCCC1

InChI

1S/C8H17NO3S/c10-13(11,12)7-6-9-8-4-2-1-3-5-8/h8-9H,1-7H2,(H,10,11,12)

InChI key

MKWKNSIESPFAQN-UHFFFAOYSA-N

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Related Categories

General description

CHES (2-(Cyclohexylamino) ethane sulfonic acid) is a derivative of amino acid taurine. It serves as a standard physiological buffer in the pH range of 8.5-9.5.

Application

CHES may be used:
  • for pH adjustments and as a assay buffer in catechol oxidase activity measurements(32)
  • as a buffer in electron paramagnetic resonance (EPR) spectroscopy and kinetic measurements(33)
  • as a buffer for soil solution equivalent (SSE) yeast dextrose medium for screening pH optima for growth of Aridibacter nitratireducens A24_SHP-5_238T(34)

Packaging

25, 100 g in poly bottle

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Hazard Codes

Xi

Risk Statement

36

Safety Statement

26

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Buffers for the physiological pH range: Acidic dissociation constants of zwitterionic compounds (ACES and CHES) in water from 5 to 55? C
Roy RN, et al.
Journal of Chemical and Engineering Data, 42(1), 41-44 (1997)
Jun Yang et al.
Langmuir : the ACS journal of surfaces and colloids, 24(18), 10265-10272 (2008-08-20)
A universal nitric oxide (NO) generating surface is assembled via Layer-by-Layer (LbL) deposition of sodium alginate (Alg) and organoselenium modified polyethyleneimine (SePEI) on quartz and polymeric substrates. The immobilized SePEI species is capable of catalytically decomposing S-nitrosothiol species (RSNO) to...
Hao Zhong et al.
The Analyst, 136(21), 4486-4491 (2011-09-29)
On-line concentration via Electrokinetic Supercharging (EKS) was used to enhance the sensitivity of the capillary electrophoretic separation of the four flavonoids naringenin, hesperetin, naringin and hesperidin. Separation conditions, including the background electrolyte pH and concentration, the length and choice of...
A D Carraway et al.
Journal of inorganic biochemistry, 60(4), 267-276 (1995-12-01)
Electron paramagnetic resonance spectra of a number of ferric heme peptide derivatives, in aqueous-detergent and various aqueous-alcohol solvent mixtures, have been obtained using samples in the concentration range 0.1-1.0 mM. Some of these were clearly monomeric, homogeneous, mixed-ligand adducts, entirely...
M G Hartmanis et al.
Proceedings of the National Academy of Sciences of the United States of America, 84(1), 76-79 (1987-01-01)
A procedure for solubilization of the oxygen-labile, membrane-bound diol dehydratase from Clostridium glycolicum with retention of enzymatic activity is described. The procedure involves sonication of crude membrane preparations anaerobically in 0.1 M 2-(N-cyclohexylamino)ethane-sulfonic acid (CHES) buffer (pH 8.6-9.0) containing 2...

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