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C8726

Sigma-Aldrich

Caspase 9 human

≥90% (SDS-PAGE), recombinant, expressed in E. coli (C-terminal histidine-tagged), buffered aqueous solution, >2,000 units/mg protein

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Synonym(s):
ICE-Lap6, Mch6
Enzyme Commission number:
3.4.22.62 (BRENDA, IUBMB)
MDL number:
MFCD02687006

recombinant

expressed in E. coli (C-terminal histidine-tagged)

Quality Level

200

Assay

≥90% (SDS-PAGE)

form

buffered aqueous solution

specific activity

>2,000 units/mg protein

mol wt

N-terminal prodomain plus the large subunit. 36 kDa (caspase 9 expressed as a C-terminal histidine-tagged protein appears as a two-subunit protein)
small subunit 13 kDa (subunit containing the histidine tag)

UniProt accession no.

P55211

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... CASP9(842)

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This Item
C1224218807SAB5700785
Sigma-Aldrich

Sigma-Aldrich

C8726

Caspase 9 human

Caspase 3 human ≥90% (SDS-PAGE), recombinant, expressed in E. coli (C-terminal histidine-tagged), buffered aqueous glycerol solution, ≥1.0 units/mg protein

Sigma-Aldrich

C1224

Caspase 3 human

Sigma-Aldrich

Sigma-Aldrich

218807

Caspase-9, Human, Recombinant, E. coli

Anti-Caspase 9 antibody produced in rabbit

Sigma-Aldrich

SAB5700785

Anti-Caspase 9 antibody produced in rabbit

assay

≥90% (SDS-PAGE)

assay

≥90% (SDS-PAGE)

assay

≥70% (SDS-PAGE)

assay

-

form

buffered aqueous solution

form

buffered aqueous glycerol solution

form

lyophilized

form

buffered aqueous solution

specific activity

>2,000 units/mg protein

specific activity

≥1.0 units/mg protein

specific activity

≥400 units/mg protein

specific activity

-

mol wt

N-terminal prodomain plus the large subunit. 36 kDa (caspase 9 expressed as a C-terminal histidine-tagged protein appears as a two-subunit protein), small subunit 13 kDa (subunit containing the histidine tag)

mol wt

~30 kDa

mol wt

-

mol wt

17, 30, 36, 46

UniProt accession no.

P55211

UniProt accession no.

P42574

UniProt accession no.

-

UniProt accession no.

P55211

Biochem/physiol Actions

When cells receive apoptotic stimuli, such as activation of the TNFα/Fas cell surface receptor, caspase 8 activation, Bid processing and its translocation to the mitochondria ensue. As a result mitochondria release cytochrome c which then binds to Apaf-1, the mammalian Ced-4 homologue, together with dATP. The resultant complex recruits caspase 9 leading to its activation. It then cleaves downstream caspases such as caspase 3, 6, and 7, initiating the caspase cascade. Caspase 9 exhibits basal activity. It can be further activated via interaction of its N-terminal prodomain with the activator protein Apaf-1 in the presence of cytochrome c and dATP. Caspases have been implicated in many disorders including cancer, inflammatory disease, neurodegenerative diseases, stroke and myocardial infarction.

Unit Definition

One unit will cleave 1.0 nmol of Ac-Leu-Glu-His-Asp-AFC per hr at 25 °C at pH 6.5.

Physical form

Solution in 10% sucrose containing 50 mM HEPES, pH 7.5, 5 mM DTT, 0.1% CHAPS, 1 mM EDTA, 50 mM NaCl.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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H R Stennicke et al.
The Journal of biological chemistry, 274(13), 8359-8362 (1999-03-20)
The recombinant form of the proapoptotic caspase-9 purified following expression in Escherichia coli is processed at Asp315, but largely inactive; however, when added to cytosolic extracts of human 293 cells it is activated 2000-fold in the presence of cytochrome c
H Duan et al.
The Journal of biological chemistry, 271(28), 16720-16724 (1996-07-12)
Members of the ICE/Ced-3 gene family are likely effector components of the cell death machinery. Here, we characterize a novel member of this family designated ICE-LAP6. By phylogenetic analysis, ICE-LAP6 is classified into the Ced-3 subfamily which includes Ced-3, Yama/CPP32/apopain
S M Srinivasula et al.
The Journal of biological chemistry, 271(43), 27099-27106 (1996-10-25)
Recent evidence suggests that CPP32 is an essential component of an aspartate-specific cysteine protease (ASCP) cascade responsible for apoptosis execution in mammalian cells. Activation of CPP32 could lead to activation of other downstream ASCPs, resulting in late morphological changes such
K Kuida
The international journal of biochemistry & cell biology, 32(2), 121-124 (2000-02-25)
Caspase-9 is a member of caspase family of cysteine proteases that have been implicated in apoptosis and cytokine processing. When cells receive apoptotic stimuli, mitochondria releases cytochrome c which then binds to Apaf-1, the mammalian Ced-4 homologue, together with dATP.
BCL-2 family members and the mitochondria in apoptosis.
A Gross et al.
Genes & development, 13(15), 1899-1911 (1999-08-13)

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