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C8854

Sigma-Aldrich

Anti-Caspase 13 (ERICE) antibody produced in rabbit

~0.5 mg/mL, IgG fraction of antiserum, buffered aqueous solution

MDL number:

biological source

rabbit

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 43 kDa

species reactivity

mouse, rat, human

concentration

~0.5 mg/mL

application(s)

microarray: suitable
western blot: 0.5-1 μg/mL using total HeLa cell lysate or rat or mouse brain lysates.

conjugate

unconjugated

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... CASP13(8628)

Immunogen

synthetic peptide corresponding to amino acids 91-105 of human caspase 13.

Application

Anti-Caspase 13 (ERICE) antibody is suitable western blot at a concentration of 0.5-1μg/mL using total HeLa cell lysate or rat or mouse brain lysates. It is also suitable for microarray.

Biochem/physiol Actions

Caspases belongs to ICE (interleukin 1β-converting enzyme) family in almost 13 different form. Caspase-13 (ERICE, evolutionary-related interleukin-1 β converting enzyme) is a recently discovered novel member of the caspase family. It is synthesized as inactive proenzymes comprising an N-terminal peptide (prodomain) including one large and one small subunit. It is expressed in a variety of human tissues and cell lines but mainly expressed in peripheral blood lymphocytes, spleen, and placenta. Overexpression of ERICE induces apoptosis of human embryonic kidney cells and breast carcinoma cells. Caspase-13 induces apoptosis. It is activated by caspase-8, which is a key enzyme in death receptor induced apoptosis.

Physical form

solution in phosphate buffered saline, containing 0.02% sodium azide.

Certificate of Analysis

Certificate of Origin

D W Nicholson et al.
Trends in biochemical sciences, 22(8), 299-306 (1997-08-01)
Caspases (cysteinyl aspartate-specific proteinases) mediate highly specific proteolytic cleavage events in dying cells, which collectively manifest the apoptotic phenotype. The key and central role that these enzymes play in a biochemical cell-suicide pathway has been conserved throughout the evolution of...
E W Humke et al.
The Journal of biological chemistry, 273(25), 15702-15707 (1998-06-23)
Programmed cell death, or apoptosis, is a process of fundamental importance to cellular homeostasis in metazoan organisms (Ellis, R. E., Yuan, J., and Horvitz, H. R. (1991) Annu. Rev. Cell Biol. 7, 663-698). The caspase family of mammalian proteases, related...
G M Cohen
The Biochemical journal, 326 ( Pt 1), 1-16 (1997-08-15)
Apoptosis is a major form of cell death, characterized initially by a series of stereotypic morphological changes. In the nematode Caenorhabditis elegans, the gene ced-3 encodes a protein required for developmental cell death. Since the recognition that CED-3 has sequence...

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