C8897

Sigma-Aldrich

Collagen from rat tail

Bornstein and Traub Type I (Sigma Type VII), powder

CAS Number:
EC Number:
MDL number:

biological source

rat tail

form

powder

application(s)

cell culture | mammalian: suitable

solubility

aqueous acid: soluble

UniProt accession no.

storage temp.

2-8°C

Gene Information

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Application

This product is intended to produce thin layer coatings on tissue culture plates to facilitate attachment of anchorage-dependent cells, recommended for use at 6-10 μg/cm2. It is NOT intended for production of 3-D gels. Type I collagen is often used in cell culture as an attachment substratum with myoblasts, spinal ganglia, hepatocytes, embryonic lung, heart explants, fibroblasts, endothelial cells, and islet cells have all been cultured successfully on films or gels of type I collagen. Collagen type I may also be used in research of Idiopathic pulmonary fibrosis (IPF), studies on the effect of ER stress IPF on lung fibroblasts. Collagen in acidic solution can produce three dimensional scaffolding with use in bioengineering and cell culture applications.

Biochem/physiol Actions

Type I collagen is a component of skin, bone, tendon, and other fibrous connective tissues.

Components

All collagen molecules are composed of three polypeptide chains arranged in a triple helical conformation, with a primary structure that is mostly a repeating motif with glycine in every third position and proline or 4-hydroxyproline frequently preceding the glycine residue. Type I collagen differs from other collagens by its low lysine hydroxylation and low carbohydrate composition.

Preparation Note

This product was prepared by a modification of the extraction method of Bornstein, M.B., Lab. Invest., 7, 134 (1958). It is an acid soluble collagen. It dissolves in water with acetic acid added to pH 3.0 at 5 mg/mL, yielding an opalescent, colorless solution.

Other Notes

Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma type designations with recognized collagen classification types.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

1

Certificate of Analysis

Certificate of Origin

Katarina Wolf et al.
The Journal of cell biology, 201(7), 1069-1084 (2013-06-27)
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Emily Hams et al.
Proceedings of the National Academy of Sciences of the United States of America, 111(1), 367-372 (2013-12-18)
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