Monoclonal Anti-Vimentin (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. Vimentin is expressed in breast myoepithelial cells, osteocytes, Langerhans cells of the skin, Schwann cells and astrocytes.
Vimentin is one of the five major groups of intermediate filaments with a molecular mass of 57 kDa. Intermediate filaments (IFs) with characteristic 10 nm diameter are a distinct class of molecularly heterogenous cytoskeletal filaments defined by ultrastructural, immunological and biochemical criteria. IFs differ significantly from the other cytoskeletal elements of the cell, namely microtubules and microfilaments and are components of most eukaryotic cells.
The antibody localizes vimentin in fibroblasts, endothelial cells, lymphoid tissue and melanocytes in immunohistochemical staining. It also stains vimentin in sarcomas, lymphomas, and melanomas.
pig eye lens vimentin.
Monoclonal Anti-Vimentin-Cy3 antibody produced in mouse has been used in:
- immunofluorescence and confocal microscopy
- cytoskeleton staining
- double labeling experiments
- immunocytochemical localozation
Cy3 Monoclonal Anti-Vimentin reacts with normal and pathological tissue of mesenchymally-derived cells, lens tissue and various cultured cells. It localizes vimentin in fibroblasts, lipocytes, endothelial cells, some lymphoid cells, melanocytes, macrophages, and chondrocytes. The antibody stains tumors derived from these cells, including sarcomas, most lymphomas, melanomas and their metastatic lesions. Co-expression of vimentin with keratin was described in most carcinomas of certain sites (e.g., kidney and thyroid) and certain carcinomas of other sites (e.g., breast).
Vimentin was found to be important for stabilizing the architecture of the cytoplasm. It was found, that activated macrophages secrete vimentin into the extracellular space in vitro.
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide
Storage and Stability
Store at 2-8 °C. Protect from prolonged exposure to light. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Cy is distributed under license from Amersham Biosciences Limited.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.