C9080

Sigma-Aldrich

Monoclonal Anti-Vimentin−Cy3 antibody produced in mouse

clone V9, purified immunoglobulin, buffered aqueous solution

Synonym(s):
Monoclonal Anti-Vimentin
NACRES:
NA.41
Pricing and availability is not currently available.

Quality Level

biological source

mouse

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

V9, monoclonal

form

buffered aqueous solution

mol wt

antigen ~58 kDa

species reactivity

chicken, rat, bovine, feline, hamster, gerbil, horse, canine, rabbit, monkey, pig, human

application(s)

direct immunofluorescence: 1:200 using cultured cells
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50 using human tonsil
immunohistochemistry (frozen sections): suitable using human tonsil

isotype

IgG1

conjugate

CY3 conjugate

UniProt accession no.

shipped in

wet ice

storage temp.

2-8°C

Gene Information

human ... VIM(7431)
rat ... Vim(81818)

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General description

Monoclonal Anti-Vimentin (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. Vimentin is expressed in breast myoepithelial cells, osteocytes, Langerhans cells of the skin, Schwann cells and astrocytes.
Vimentin is one of the five major groups of intermediate filaments with a molecular mass of 57 kDa. Intermediate filaments (IFs) with characteristic 10 nm diameter are a distinct class of molecularly heterogenous cytoskeletal filaments defined by ultrastructural, immunological and biochemical criteria. IFs differ significantly from the other cytoskeletal elements of the cell, namely microtubules and microfilaments and are components of most eukaryotic cells.

Specificity

The antibody localizes vimentin in fibroblasts, endothelial cells, lymphoid tissue and melanocytes in immunohistochemical staining. It also stains vimentin in sarcomas, lymphomas, and melanomas.

Immunogen

pig eye lens vimentin.

Application

Monoclonal Anti-Vimentin-Cy3 antibody produced in mouse has been used in:
  • immunofluorescence and confocal microscopy
  • cytoskeleton staining
  • double labeling experiments
  • immunohistochemical
  • immunocytochemical localozation

Biochem/physiol Actions

Cy3 Monoclonal Anti-Vimentin reacts with normal and pathological tissue of mesenchymally-derived cells, lens tissue and various cultured cells. It localizes vimentin in fibroblasts, lipocytes, endothelial cells, some lymphoid cells, melanocytes, macrophages, and chondrocytes. The antibody stains tumors derived from these cells, including sarcomas, most lymphomas, melanomas and their metastatic lesions. Co-expression of vimentin with keratin was described in most carcinomas of certain sites (e.g., kidney and thyroid) and certain carcinomas of other sites (e.g., breast).
Vimentin was found to be important for stabilizing the architecture of the cytoplasm. It was found, that activated macrophages secrete vimentin into the extracellular space in vitro.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Storage and Stability

Store at 2-8 °C. Protect from prolonged exposure to light. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Legal Information

Cy is distributed under license from Amersham Biosciences Limited.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Cancer cell motility is affected through 3D cell culturing and SCF/c-Kit pathway but not by X-irradiation
Eberle F, et al.
Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology, 119(3) (2016)
Endometrial stromal and epithelial cells exhibit unique aberrant molecular defects in patients with endometriosis
Logan PC, et al.
Reproductive Sciences, 25(1) (2018)
HaYeun Ji et al.
Biomaterials, 85, 180-194 (2016-02-14)
Access to smooth muscle cells (SMC) would create opportunities for tissue engineering, drug testing, and disease modeling. Herein we report the direct conversion of human endothelial progenitor cells (EPC) to induced smooth muscle cells (iSMC) by induced expression of MYOCD....
Morphology of the myoepithelial cell: immunohistochemical characterization from resting to motile phase
Beha G, et al.
TheScientificWorldJournal, 2012 (2012)
Coexpression of keratin-and vimentin-type intermediate filaments in human metastatic carcinoma cells
Ramaekers FC, et al.
Proceedings of the National Academy of Sciences of the USA, 80(9), 2618-2622 (1983)

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