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C9210

Sigma-Aldrich

Cyanogen bromide-activated Agarose

lyophilized powder

CAS Number:
MDL number:
NACRES:
NA.56

form

lyophilized powder

Quality Level

matrix

cross-linked 4% beaded agarose

matrix active group

cyanate or related structures

matrix spacer

1 atom (when ligands are coupled via isourea derivative or related linkage)

swelling

1 g swells to 2.5-4.5 mL

capacity

≥10 mg/mL binding capacity (BSA)(via amino groups)

storage temp.

−20°C

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Application

Cyanogen bromide-activated Agarose is lyophilized powder stabilized with lactose used in affinity chromatography, protein chromatography, protein interactions, antibody labeling, antibody modification and attaching antibodies to agarose beads.

Packaging

1, 5, 15 g in poly bottle

Physical form

Lyophilized powder stabilized with lactose

Storage Class Code

13 - Non Combustible Solids

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What do you recommend to use as a negative control for Product C9210, Cyanogen bromide?

    The control for this product is Product No. CL4B200 (Sepharose CL-4B).

  4. What is the difference between Product C9210, Cyanogen bromide and Product C9142, Cyanogen bromide-activated-Sepharose® 4B?

    Sigma-Aldrich produces product C9210, while C9142 is produced by GE Healthcare.  In addition, C9210 is a cross-linked resin and C9142 is not.

  5. When using Product C9210, Cyanogen bromide, why must amine containing reagents be avoided during coupling?

    The ligand is attached to the resin via an amine group. If an amine-containing buffer is used during coupling, you will greatly reduce the amount of ligand that can attach.

  6. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

DamIP: Using Mutant DNA Adenine Methyltransferase to Study DNA-Protein Interactions <I>In Vivo</I>.
Xiao, R., and Moore, D.D.
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C A Schall et al.
Biotechnology and bioengineering, 53(1), 41-48 (1997-01-05)
The stability of NAD(H) immobilized to a crosslinked agarose support (Sepharose(R)-4B) was examined in buffer solutions at a pH of 7.0 and 8.5. Specifically, this study investigated particle attrition and ligand leakage rates from a cyanogen bromide activated agarose support....
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Mi Young Cha et al.
Bioconjugate chemistry, 22(1), 88-94 (2010-12-15)
We developed fluorescent biosensor systems that are either general or selective to fluoroquinolone antibiotics by using a single-chain variable-fragment (scFv) as a recognition element. The selectivity of these biosensors to fluoroquinolone antibiotics was rationally tuned through the structural modification on...

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