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C9281

Sigma-Aldrich

Cholesterol Esterase from Pseudomonas fluorescens

lyophilized powder, ≥10,000 units/g protein

Synonym(s):
Sterol-ester acylhydrolase
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
NACRES:
NA.54

form

lyophilized powder

Quality Level

specific activity

≥10,000 units/g protein

mol wt

~129 kDa

composition

Protein, ~20%

solubility

0.4 M potassium phosphate, pH 7.0: soluble 1.0 mg/mL

storage temp.

−20°C

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Application

This enzyme is widely used in the determination of serum cholesterol in diagnostic laboratories.
Cholesterol esterase from Pseudomonas fluorescens has been used in an optimization study of components in enzymatic cholesterol reagents containing cholesterol oxidase. Cholesterol esterase from Pseudomonas fluorescens has also been used in a study to investigate the nondenaturing protein electrotransfer of the esterase activity of lipolytic preparations.

Packaging

100, 500 units in poly bottle

Biochem/physiol Actions

Cholesterol esterase (CE) is a reversible enzyme that can hydrolyze or synthesize fatty acid esters of cholesterol and other sterols. Hydrolysis of water insoluble long chain fatty acid esters requires bile salt activation. Hydrolysis of water soluble esters of short chain fatty acids and lysophospholipids does not require activation by bile salts. It also hydrolyzes tri-, di-, and mono-acylglycerols, phospholipids, lysophospholipids, and ceramides. The enzyme may have multiple functions in lipid and lipoprotein metabolism, as well as in atherosclerosis. Its molecular mass is approximately 129 kDa and the optimum pH range is 7.0-9.0. The enzyme is activated by cholic acid, glycocholic acid, BSA, Mg2+ and 0.3% (v/v) X-100. Ag+, Hg2+ and ionic detergents inhibit the activity of the enzyme.

Other Notes

Contains potassium phosphate and TRITON® X-100.

Unit Definition

One unit will hydrolyze 1.0 μmole of cholesteryl oleate to cholesterol and oleic acid per min at pH 7.0 at 37 °C in the presence of taurocholate.

Analysis Note

Protein determined by biuret.

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Certificate of Origin

Carolina Espinosa Álvarez et al.
Molecules (Basel, Switzerland), 25(24) (2020-12-30)
Haematococcus pluvialis is the largest producer of natural astaxanthin in the world. Astaxanthin is a bioactive compound used in food, feed, nutraceutics, and cosmetics. In this study, astaxanthin extraction from H. pluvialis by supercritical fluid extraction was evaluated. The effects
David Y Hui et al.
Journal of lipid research, 43(12), 2017-2030 (2002-11-28)
Carboxyl ester lipase (CEL), previously named cholesterol esterase or bile salt-stimulated (or dependent) lipase, is a lipolytic enzyme capable of hydrolyzing cholesteryl esters, tri-, di-, and mono-acylglycerols, phospholipids, lysophospholipids, and ceramide. The active site catalytic triad of serine-histidine-aspartate is centrally
Qiang Li et al.
Pflugers Archiv : European journal of physiology, 465(9), 1303-1316 (2013-04-23)
Nonalcoholic fatty liver disease (NAFLD) is characterized by hepatic lipid deposition and coincides often with cardiometabolic diseases. Several dietary factors attenuate NAFLD. Here, we report beneficial effects of chronic dietary capsaicin intake on NAFLD which is mediated by the transient
Tri Duc Ngo et al.
Acta crystallographica. Section D, Biological crystallography, 69(Pt 9), 1726-1737 (2013-09-04)
Intracellular mobilization of fatty acids from triacylglycerols in mammalian adipose tissues proceeds through a series of lipolytic reactions. Among the enzymes involved, hormone-sensitive lipase (HSL) is noteworthy for its central role in energy homeostasis and the pathogenic role played by
Nathan O Stitziel et al.
Arteriosclerosis, thrombosis, and vascular biology, 33(12), 2909-2914 (2013-09-28)
Autosomal recessive hypercholesterolemia is a rare inherited disorder, characterized by extremely high total and low-density lipoprotein cholesterol levels, that has been previously linked to mutations in LDLRAP1. We identified a family with autosomal recessive hypercholesterolemia not explained by mutations in

Articles

Cholesterol Esterification

Cholesterol undergoes esterification to improve transport. Cholesterol esters are more easily packaged into the interior of lipoproteins - increasing the quantity that can be readily transported in the blood stream.

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