C9301

Sigma-Aldrich

Collagen from chicken sternal cartilage

Type II (Miller), powder, BioReagent, suitable for cell culture

CAS Number:
EC Number:
MDL number:
NACRES:
NA.75

Quality Level

biological source

chicken (Sternal cartilage)

sterility

non-sterile

type

Type II (Miller)

product line

BioReagent

assay

60-80% (biuret)

form

powder

packaging

glass bottle of 100 mg
poly bottle of 25 mg
glass bottle of 5 mg

application(s)

cell culture | mammalian: suitable

surface coverage

6‑10 μg/cm2

solubility

acetic acid: 0.5-2.0 mg/mL (Dissolve for several hours at 2-8 °C, occasionally swirling.)

UniProt accession no.

shipped in

wet ice

storage temp.

2-8°C

Gene Information

chicken ... COL2A1(395069)

Related Categories

General description

Collagen is made of three polypeptide chains. Type 2 collagen is ubiquitously present in the hyaline cartilage. It is a homotrimer made of three α1 chains. Type 2 collagen is also predominant in nucleus pulposus of the vertebral disc and vitreous body of the eye. The proteoglycan obtained from the chicken sternal cartilage is similar to type nine collagen structure.
Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma type designations with recognized collagen classification types.

Application

Collagen from chicken sternal cartilage has been used in enzyme–linked immunosorbent assay (ELISA) , chondrocyte-mediated tissue production in vitro, induction, treatment and assessment of collagenα induced arthritis (CIA) and cell proliferation assay.

Packaging

25 mg in poly bottle
5 mg in glass bottle
100 mg in glass bottle

Other Notes

Major component of articular cartilage.

Preparation Note

Prepared by a modification of the method of Trentham, D.E., et al.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Mesenchymal-Epithelial Interactions in Neural Development (2013)
Cytokine profiles in the joint depend on pathology, but are different between synovial fluid, cartilage tissue and cultured chondrocytes.
Tsuchida A I, et al.
Arthritis Research & Therapy, 16(5), 441-441 (2014)
Biology of Proteoglycans (2013)
High-density lipoproteins attenuate high glucose-impaired endothelial cell signaling and functions: potential implications for improved vascular repair in diabetes.
Chen X, et al.
Cardiovascular Diabetology, 16(1), 121-121 (2017)
Deletion of either CD55 or CD97 ameliorates arthritis in mouse models.
Hoek R M, et al.
Arthritis and Rheumatism, 62(4), 1036-1042 (2010)
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