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MilliporeSigma

C9426

Sigma-Aldrich

CHAPS hydrate

BioReagent, suitable for electrophoresis, ≥98% (HPLC)

Synonym(s):

3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate hydrate, CHAPS

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About This Item

Empirical Formula (Hill Notation):
C32H58N2O7S · xH2O
CAS Number:
Molecular Weight:
614.88 (anhydrous basis)
MDL number:
UNSPSC Code:
12161902
PubChem Substance ID:
NACRES:
NB.22

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$79.89

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In StockDetails


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description

zwitterionic

Quality Level

product line

BioReagent

assay

≥98% (HPLC)

form

powder

mol wt

614.88 g/mol (anhydrous basis)

technique(s)

electrophoresis: suitable
protein quantification: suitable

impurities

<0.04% DMF

mp

157 °C (dec.) (lit.)

solubility

water: 50 mg/mL, clear, colorless

suitability

suitable for electrophoresis

storage temp.

room temp

SMILES string

O.C[C@H](CCC(=O)NCCC[N+](C)(C)CCCS([O-])(=O)=O)[C@H]1CC[C@H]2[C@@H]3[C@H](O)C[C@@H]4C[C@H](O)CC[C@]4(C)[C@H]3C[C@H](O)[C@]12C

InChI

1S/C32H58N2O7S.H2O/c1-21(8-11-29(38)33-14-6-15-34(4,5)16-7-17-42(39,40)41)24-9-10-25-30-26(20-28(37)32(24,25)3)31(2)13-12-23(35)18-22(31)19-27(30)36;/h21-28,30,35-37H,6-20H2,1-5H3,(H-,33,38,39,40,41);1H2/t21-,22+,23-,24-,25+,26+,27-,28+,30+,31+,32-;/m1./s1

InChI key

SJCUTFKCLFLIFE-JWTJKVBLSA-N

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1 of 4

This Item
C3023C5070220201
technique(s)

electrophoresis: suitable, protein quantification: suitable

technique(s)

dialysis: suitable, electrophoresis: suitable

technique(s)

protein purification: suitable, protein quantification: suitable

technique(s)

-

assay

≥98% (HPLC)

assay

≥98% (HPLC)

assay

≥98% (TLC)

assay

≥98% (NMR)

Quality Level

200

Quality Level

400

Quality Level

400

Quality Level

300

product line

BioReagent

product line

-

product line

BioXtra

product line

-

form

powder

form

powder

form

powder

form

crystalline powder

storage temp.

room temp

storage temp.

2-30°C

storage temp.

-

storage temp.

15-25°C

General description

CHAPS is a non-denaturing, zwitterionic sulfobetaine detergent. It is mild and solubilizes membrane proteins and receptors. It is useful for breaking protein-protein interactions. It is a derivative of the naturally occurring bile salts.[1] It has small micellar molecular weight (6,150) and high critical micellar concentration (6 - 10 mM) allowing it to be removed from samples by dialysis.

Application

CHAPS has been used:
  • For membrane permeabilization of cells[2]
  • As a component in lysis buffer for homogenization of liver tissues as well as adipose tissue[3][4]
  • As a component in denaturing buffer in enzyme characterization[5]
  • As a component of 2D- sample buffer in 2D electrophoresis[6]
  • For protein solubilization in isoelectric focusing[7]

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Acute Tox. 4 Oral

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Questions

1–3 of 3 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. How do I store CHAPS, once it's dissolved in H2O?

    1 answer
    1. Solutions prepared at 100 mM in water are stable for at least 1 year when stored refrigerated. Note that a prepared solution is available. Please see the link below to review:
      https://www.sigmaaldrich.com/product/sigma/19899

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