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C9584

Sigma-Aldrich

Carboxypeptidase B from porcine pancreas

lyophilized powder

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Synonym(s):
Peptidyl-L-lysine(L-arginine) hydrolase, Protaminase
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
PubChem Substance ID:

biological source

Porcine pancreas

Quality Level

grade

Proteomics Grade

form

lyophilized powder

specific activity

≥125 units/mg protein

mol wt

34,000 Da± 600

purified by

affinity chromatography

composition

Protein, 40-70%

foreign activity

carboxypeptidase A ≤1%
chymotrypsin ≤0.1%
trypsin ≤5%

storage temp.

−20°C

InChI

1S/C31H38N4O7S/c1-20(32-26(36)15-16-27(37)38)28(39)33-21(2)30(41)35-17-9-14-25(35)29(40)34-24(18-22-10-5-3-6-11-22)31(42)43-19-23-12-7-4-8-13-23/h3-8,10-13,20-21,24-25H,9,14-19H2,1-2H3,(H,32,36)(H,33,39)(H,34,40)(H,37,38)

InChI key

TWURVFFNODFJBJ-UHFFFAOYSA-N

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This Item
C5233C9268C9658
grade

Proteomics Grade

grade

-

grade

Proteomics Grade

grade

-

form

lyophilized powder

form

solution

form

ready-to-use solution

form

lyophilized powder

specific activity

≥125 units/mg protein

specific activity

50-55 units/mg protein carboxypeptidase B

specific activity

≥50 units/mg protein

specific activity

≥3 units/mg protein

mol wt

34,000 Da± 600

mol wt

-

mol wt

~35 kDa

mol wt

homodimer ~90 kDa

purified by

affinity chromatography

purified by

-

purified by

2× crystallization

purified by

-

Application

Carboxypeptidase B has been used in a study to develop a non-invasive pregnancy assay for use in both captive and wild polar bears. Carboxypeptidase B has been used in a study that identified new potential biomarkers of acute pancreatitis.
The enzyme from Sigma has been used to develop homogeneous time-resolved fluorescence (HTRF) assay for measuring carboxypeptidase B activity in a miniaturized high-throughput screening format. It has been used to evaluate the impact of the C-terminal lysine(s) in human plasminogen binding to Bifidobacterium. The effect of treatment with carboxypeptidase B, which is a C-terminal lysine-specific endopeptidase, is measured using flow cytometry analysis.

Biochem/physiol Actions

Carboxypeptidase B is a proteolytic enzyme capable of rapidly hydrolyzing peptide bonds to release certain carboxyl-terminal basic amino acids from peptides and proteins. Its molecular mass is 34,300±600 Da. It contains one non-dialyzable gram atom of zinc per mole. The enzyme activity is inhibited by metal chelating agents 1, 10-phenanthroline, 8-hydroxyquinoline-5-sulfonic acid, and 2,2′-dipyridyl.

Packaging

Package size based on protein content

Unit Definition

One unit will hydrolyze 1.0 μmole of hippuryl-L-arginine per min at pH 7.65 at 25 °C.

Physical form

Contains HEPES buffer salts, zinc chloride and carbohydrate

Preparation Note

Treated with protease inhibitor, AEBSF, to eliminate serine protease activity.

Analysis Note

Protein determined by biuret.

Pictograms

Exclamation markHealth hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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E Curry et al.
Theriogenology, 78(2), 308-314 (2012-04-28)
Currently, there is no method of accurately and non-invasively diagnosing pregnancy in polar bears. Specific proteins may exhibit altered profiles in the feces of pregnant bears, but predicting appropriate candidate proteins to investigate is speculative at best. The objective of
Marc Ferrer et al.
Analytical biochemistry, 317(1), 94-98 (2003-05-06)
An epitope-unmasking, homogeneous time-resolved fluorescence (HTRF) assay has been developed for measuring carboxypeptidase B (CPB) activity in a miniaturized high-throughput screening format. The enzyme substrate (biotin-RYRGLMVGGVVR-OH) is cleaved by CPB at the C terminus, causing release of the C-terminal Arg
Marco Candela et al.
Journal of bacteriology, 189(16), 5929-5936 (2007-06-15)
Bifidobacteria constitute up to 3% of the total microbiota and represent one of the most important health-promoting bacterial groups of the human intestinal microflora. The presence of Bifidobacterium in the human gastrointestinal tract has been directly related to several health-promoting
Laboratory diagnosis of acute pancreatitis: in search of the Holy Grail.
Lippi, G. et al.
Clinical Laboratory Science : Journal of the American Society For Medical Technology, 49, 18-31 (2012)
Anna Rozhkova
Journal of chromatography. A, 1216(32), 5989-5994 (2009-06-30)
A robust cation-exchange chromatofocusing method was developed for the routine analysis of a recombinant humanized monoclonal IgG antibody. We compare the chromatofocusing method to the conventional cation-exchange chromatography (CEX) employing a salt gradient and show clear advantages of chromatofocusing over

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