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C9658

Carboxypeptidase G from Pseudomonas sp.

lyophilized powder, ≥3 units/mg protein

Synonym(s):

γ-Glutamyl hydrolase

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$270.00

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$804.00

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About This Item

CAS Number:
9074-87-7
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-978-1
MDL number:
MFCD00130719
EC Number:
3.4.17.11 (BRENDA, IUBMB)
Specific activity:
≥3 units/mg protein

Key Documents

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form

lyophilized powder

specific activity

≥3 units/mg protein

mol wt

homodimer ~90 kDa

composition

Protein, ~70% biuret

solubility

H2O: soluble 0.5 mg/mL

storage temp.

−20°C

Quality Level

300

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Carboxypeptidase G from Pseudomonas sp. lyophilized powder, ≥3 units/mg protein

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Carboxypeptidase Y from baker’s yeast (S. cerevisiae) lyophilized powder, ≥50 units/mg protein

Sigma-Aldrich

C3888

Carboxypeptidase Y from baker′s yeast (S. cerevisiae)

specific activity

≥3 units/mg protein

specific activity

≥125 units/mg protein

specific activity

≥50 units/mg protein

specific activity

≥50 units/mg protein

form

lyophilized powder

form

lyophilized powder

form

ready-to-use solution

form

lyophilized powder

mol wt

homodimer ~90 kDa

mol wt

34,000 Da± 600

mol wt

~35 kDa

mol wt

61 kDa

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

solubility

H2O: soluble 0.5 mg/mL

solubility

-

solubility

-

solubility

-

Quality Level

300

Quality Level

200

Quality Level

300

Quality Level

200

Application

Carboxypeptidase G from Pseudomonas sp., or γ-Glutamyl hydrolase, has been used in a study to assess the role of the putidaredoxin COOH-terminus in P-450cam (cytochrome m) hydroxylations. Carboxypeptidase G from Pseudomonas sp. has also been used in a study to investigate the effects of nitric oxide on pemetrexed cytotoxicity via NO‑cGMP signaling in lung adenocarcinoma cells.

Biochem/physiol Actions

Carboxypeptidase G is a lysosomal, thiol-dependent protease, which progressively cleaves γ-glutamyl pteroyl poly-γ-glutamate yielding pteroyl-α-glutamate (folic acid) and free glutamate. It is considered highly specific for the γ-glutamyl bond, but not for the C-terminal amino acid of the leaving group.[1] Molecular mass of this homodimer is approximately 90 kDa. The enzyme is activated by Zn2+ ions.

Physical form

Contains sodium acetate salt.

Preparation Note

Chromatographically purified
Solutions should be prepared fresh prior to use.

Other Notes

One unit will hydrolyze 1.0 μmole of L-glutamic acid from (+)amethopterin per min at pH 7.3 at 30 °C.

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000458762
0000458762
0000458761
0000458761
0000453351

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The enzymatic hydrolysis of methotrexate and folic acid.
C C Levy et al.
The Journal of biological chemistry, 242(12), 2933-2938 (1967-06-25)
Edgar M Pera et al.
Gene expression patterns : GEP, 3(2), 147-152 (2003-04-25)
Endoderm development is an area of intense interest in developmental biology, but progress has been hampered by the lack of specific markers for differentiated endodermal cells. In an unbiased secretion cloning screen of Xenopus gastrula embryos we isolated a novel
Takashi Tsukamoto et al.
Bioorganic & medicinal chemistry letters, 12(16), 2189-2192 (2002-07-20)
Phosphonate and phosphinate analogues of N-acylated gamma-glutamylglutamate were tested for the ability to inhibit glutamate carboxypeptidase II (GCP II). All of the compounds inhibit GCP II with IC(50) values in the low nanomolar range. The comparison of the results to
Masaharu Suzuki et al.
Current opinion in plant biology, 11(5), 548-553 (2008-08-12)
Two subfamilies of plant-specific B3 domain transcription factors regulate the fundamental transition between seed and vegetative phases of development. The AFL B3 genes activate the embryo maturation program, while the closely related VAL B3 genes shutdown the AFL network before
Jeremy P Mallari et al.
Bioorganic & medicinal chemistry, 12(22), 6011-6020 (2004-10-23)
A series of alkyl and aryl phosphonyl, thiophosphonyl, and dithiophosphonyl derivatives of (S)- and (R)-glutamic acid were prepared and examined for inhibitory potency against glutamate carboxypeptidase (carboxypeptidase G). The acquisition of the phosphonamidodithioic acids and the individual phosphonamidothioic acid diastereomers
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