C9903

Cholera Toxin B subunit

≥95% (SDS-PAGE), lyophilized powder

• Synonym(s): CTB
• MDL number: MFCD02356522
• PubChem Substance ID: 329775212
• NACRES: NA.77

Properties

• Quality Level: 300
• assay: ≥95% (SDS-PAGE)
• form: lyophilized powder
• mol wt: ~12 kDa
• composition: Protein, ~5% Lowry
• impurities: ≤0.5% Cholera toxin A subunit (SDS-PAGE)
• solubility: H₂O: 10 mg/mL
• storage temp.: 2-8°C
• SMILES string: CCOc1ccccc1C(=O)Nc2ccc(Cl)c(c2)C(F)(F)F
• InChI: 1S/C16H13ClF3NO2/c1-2-23-14-6-4-3-5-11(14)15(22)21-10-7-8-13(17)12(9-10)16(18,19)20/h3-9H,2H2,1H3,(H,21,22)
• InChI key: YDXZSNHARVUYNM-UHFFFAOYSA-N

Description

General description

Cholera Toxin is a heterohexameric AB5 enterotoxin. Cholera Toxin B subunit is the the B subunit pentamer of cholera toxin produced by Vibrio cholera. It is generally involved in the cell trafficking and signalling machineries.

Application

Cholera Toxin B subunit has been used:

• for macrophage stimulation and i.p. injection in a study to determine the endotoxin sensitivity of Caspase-4.
• in transganglionic and retrograde tract-tracing method combined with dual-immunofluorescence histochemistry of adult rat Vmes neuron cells.

Biochem/physiol Actions

The cholera toxin B subunit is used for track tracing in neurological research, taking advantage of GM1 ganglioside binding and retrograde transport. Tissue culture cells treated with cholera toxin are not killed and tissues of animals do not become necrotic.

Features and Benefits

This compound is a featured product for Cyclic Nucleotide research. Click here to discover more featured Cyclic Nucleotide products. Learn more about bioactive small molecules for other areas of research at sigma.com/discover-bsm.

Packaging

Package size based on protein content

Physical form

Lyophilized powder containing Tris buffer salts, sodium chloride, sodium azide, and sodium EDTA.

Reconstitution

When reconstituted with water to a final concentration of 1 mg of CTB per ml, the solution will contain 0.05 M Tris buffer, pH 7.5, 0.2 M NaCl, 3 mM NaN₃ and 1 mM sodium EDTA.

Analysis Note

Activity measured by ELISA using ganglioside GM₁-coated multiwell plates, rabbit anti-Cholera toxin B subunit antibodies, and peroxidase-labeled goat anti-rabbit IgG as the secondary antibody. 50% saturation of binding was achieved with 0.05-1 μg of Cholera toxin B subunit per mL.

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H312 + H332 - H412
• Precautionary Statements: P261 - P271 - P273 - P280 - P302 + P352 + P312 - P304 + P340 + P312
• Hazard Classifications: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Chronic 3
• Storage Class Code: 11 - Combustible Solids
• WGK Germany: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable