Skip to Content
MilliporeSigma

C9903

Sigma-Aldrich

Cholera Toxin B subunit

≥95% (SDS-PAGE), lyophilized powder, enterotoxin

Synonym(s):

CTB

Slide 1 of 1
Sign Into View Organizational & Contract Pricing

Select a Size

0.5 MG
$233.00
1 MG
$405.00
2 MG
$698.00

$233.00


Check Cart for Availability

Request a Bulk Order

Select a Size

Change View
0.5 MG
$233.00
1 MG
$405.00
2 MG
$698.00

About This Item

MDL number:
UNSPSC Code:
12352200
PubChem Substance ID:
NACRES:
NA.77

Skip To

Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

$233.00


Check Cart for Availability

Request a Bulk Order
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Product Name

Cholera Toxin B subunit, ≥95% (SDS-PAGE), lyophilized powder

Quality Level

assay

≥95% (SDS-PAGE)

form

lyophilized powder

mol wt

~12 kDa

composition

Protein, ~5% Lowry

impurities

≤0.5% Cholera toxin A subunit (SDS-PAGE)

solubility

H2O: 10 mg/mL

storage temp.

2-8°C

SMILES string

CCOc1ccccc1C(=O)Nc2ccc(Cl)c(c2)C(F)(F)F

InChI

1S/C16H13ClF3NO2/c1-2-23-14-6-4-3-5-11(14)15(22)21-10-7-8-13(17)12(9-10)16(18,19)20/h3-9H,2H2,1H3,(H,21,22)

InChI key

YDXZSNHARVUYNM-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
C3741C9972C1655
assay

≥95% (SDS-PAGE)

assay

-

assay

-

assay

-

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

Quality Level

300

Quality Level

200

Quality Level

200

Quality Level

200

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

mol wt

~12 kDa

mol wt

pentamer 57 kDa, ~12 kDa

mol wt

~12 kDa

mol wt

~12 kDa

solubility

H2O: 10 mg/mL

solubility

H2O: soluble 10 mg/mL

solubility

-

solubility

-

General description

Cholera Toxin is a heterohexameric AB5 enterotoxin. Cholera Toxin B subunit is the the B subunit pentamer of cholera toxin produced by Vibrio cholera.[1] It is generally involved in the cell trafficking and signalling machineries.[2]

Application

Cholera Toxin B subunit has been used:
  • for macrophage stimulation and i.p. injection in a study to determine the endotoxin sensitivity of Caspase-4.[3]
  • in transganglionic and retrograde tract-tracing method combined with dual-immunofluorescence histochemistry of adult rat Vmes neuron cells.[4]

Biochem/physiol Actions

The cholera toxin B subunit is used for track tracing in neurological research, taking advantage of GM1 ganglioside binding and retrograde transport.
The cholera toxin B subunit is used for track tracing in neurological research, taking advantage of GM1 ganglioside binding and retrograde transport. Tissue culture cells treated with cholera toxin are not killed and tissues of animals do not become necrotic.

Features and Benefits

This compound is a featured product for Cyclic Nucleotide research. Click here to discover more featured Cyclic Nucleotide products. Learn more about bioactive small molecules for other areas of research at sigma.com/discover-bsm.

Packaging

Package size based on protein content

Physical form

Lyophilized powder containing Tris buffer salts, sodium chloride, sodium azide, and sodium EDTA.

Reconstitution

When reconstituted with water to a final concentration of 1 mg of CTB per ml, the solution will contain 0.05 M Tris buffer, pH 7.5, 0.2 M NaCl, 3 mM NaN3 and 1 mM sodium EDTA.

Analysis Note

Activity measured by ELISA using ganglioside GM1-coated multiwell plates, rabbit anti-Cholera toxin B subunit antibodies, and peroxidase-labeled goat anti-rabbit IgG as the secondary antibody. 50% saturation of binding was achieved with 0.05-1 μg of Cholera toxin B subunit per mL.

pictograms

Exclamation mark

signalword

Warning

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Chronic 3

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Questions

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service