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CAS9BST

Sigma-Aldrich

CAS9 Blasticidin Lenti Plasmid

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NACRES:
NA.51

recombinant

expressed in E. coli

Quality Level

200

packaging

vial of 50 μL

concentration

20 ng/μL in TE buffer; DNA (1μg of plasmid DNA)

selection

ampicillin
blasticidin

shipped in

dry ice

storage temp.

−20°C

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CAS9HYGROVP

Cas9 Hygromycin Lenti Plasmid

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

-

recombinant

expressed in E. coli

packaging

vial of 50 μL

packaging

vial of 50 μL

packaging

vial of 200 μL

packaging

vial of 50 μL

concentration

20 ng/μL in TE buffer; DNA (1μg of plasmid DNA)

concentration

20 ng/μL in TE buffer; DNA (1μg of Plasmid DNA)

concentration

≥1 x 10 6 VP/ml virus (via p24 assay)

concentration

20 ng/μL in TE buffer; DNA (1μg of plasmid DNA)

selection

ampicillin

selection

ampicillin

selection

blasticidin

selection

blasticidin

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−70°C

storage temp.

−20°C

General description

This product is a blasticidin resistant lenti-Cas9 plasmid for generation of lentiviral particles. Use Sigma′s lentiviral Cas9 reagents to efficiently generate stable cell lines expressing Cas9 for CRISPR genome editing. Sigma′s lenti-Cas9 plasmid is one part of a two-vector CRISPR system with individual Cas9 and gRNA expression vectors. Please see the link below to order gRNA.

Application

Functional Genomics/Screening /Target Validation/Genome Editing

Components

1ug of plasmid DNA, EF1a-Cas9-2A-Blasticidin Lenti

Linkage

Click here to order gRNA plasmid and virus.

Storage Class Code

12 - Non Combustible Liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

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