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CAS9BST

Sigma-Aldrich

CAS9 Blasticidin Lenti Plasmid

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NACRES:
NA.51

recombinant

expressed in E. coli

Quality Level

packaging

vial of 50 μL

concentration

20 ng/μL in TE buffer; DNA (1μg of plasmid DNA)

selection

ampicillin
blasticidin

shipped in

dry ice

storage temp.

−20°C

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LVCAS9NEODCAS9P300VPCAS9HYGROV
vibrant-m

CAS9BST

CAS9 Blasticidin Lenti Plasmid

vibrant-m

LVCAS9NEO

Cas9 Neomycin Lenti Particles

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

storage temp.

−20°C

storage temp.

−70°C

storage temp.

−20°C

storage temp.

−70°C

packaging

vial of 50 μL

packaging

vial of 200 μL

packaging

vial of 50 μL

packaging

vial of 200 μL

concentration

20 ng/μL in TE buffer; DNA (1μg of plasmid DNA)

concentration

≥1 x 10 6 VP/ml virus (via p24 assay)

concentration

20 ng/μL in TE buffer; DNA (1μg of plasmid DNA)

concentration

≥1 x 10 6 VP/ml virus (via p24 assay)

selection

ampicillin

selection

neomycin / G418

selection

-

selection

blasticidin

General description

This product is a blasticidin resistant lenti-Cas9 plasmid for generation of lentiviral particles. Use Sigma′s lentiviral Cas9 reagents to efficiently generate stable cell lines expressing Cas9 for CRISPR genome editing. Sigma′s lenti-Cas9 plasmid is one part of a two-vector CRISPR system with individual Cas9 and gRNA expression vectors. Please see the link below to order gRNA.

Application

Functional Genomics/Screening /Target Validation/Genome Editing

Components

1ug of plasmid DNA, EF1a-Cas9-2A-Blasticidin Lenti

Linkage

Click here to order gRNA plasmid and virus.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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