CAS9D10AGFPP
CMV-CAS9D10A-2A-GFP Plasmid
Promotion Available
Synonym(s):
CAS9D10A Plasmid
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| Size/SKU | Availability | Price |
|---|---|---|
1 ea | Usually ships in 3 weeks. | $276.00 |
About This Item
NACRES:
NA.51
UNSPSC Code:
12352200
Promoter:
Promoter name: CMV
Reporter gene:
GFP
recombinant
expressed in E. coli
Quality Segment
form
liquid
packaging
vial of 50 μL
concentration
20 ng/μL in TE buffer; DNA (1μg of plasmid DNA)
promoter
Promoter name: CMV
reporter gene
GFP
selection
kanamycin
shipped in
dry ice
storage temp.
−20°C
General description
The Cas9-D10A nickase plasmid co-expressing GFP uses the CMV promoter for strong transient expression of Cas9-D10A. Alternate promoters can be substituted by replacement of CMV using MluI and NheI. Also, the Cas9D10A expression plasmids can be linearized using XbaI for T7-based mRNA production.
Application
Functional Genomics
Use of Paired Cas9 Nickase + GFP for:
Use of Paired Cas9 Nickase + GFP for:
- Creation of gene knockouts in multiple cell lines
- Complete knockout of genes not amenable to RNAi
- Creation of knock-in cell lines with promoters, fusion tags or reporters integrated into endogenous genes
CMV-CAS9D10A-2A-GFP Plasmid has been used in CRISPR-Cas9 mutagenesis to truncate the forkhead box O3 (FOXO3) gene in the mammalian cell.
Physical form
1 ug of Sigma Cas9-D10A nickase-GFP plasmid
Other Notes
1 vial containing 1ug of Cas9-D10A Nickase-GFP plasmid.
Please note, this product does not contain any guide RNA sequence. A pair of gRNA plasmids must be purchased separately through the Custom CRISPR paired nickase product tab.
Please note, this product does not contain any guide RNA sequence. A pair of gRNA plasmids must be purchased separately through the Custom CRISPR paired nickase product tab.
The type II CRISPR/Cas system from the bacterium Streptococcus pyogenes has been engineered to function in eukaryotic systems using two molecular components: a single Cas9 protein and a non-coding guide RNA (gRNA). The Cas9 endonuclease can be programmed with a single gRNA, directing a DNA double-strand break (DSB) at a desired genomic location. Similar to DSBs induced by zinc finger nucleases (ZFNs), the cell then activates endogenous DNA repair processes, either non-homologous end joining (NHEJ) or homology-directed repair (HDR), to heal the targeted DSB. GFP is co-expressed from the same mRNA as the Cas9 nickase protein via a 2A peptide linkage, enabling tracking of transfection efficiency and enrichment of genome editing activity in cell populations via fluorescence activated cell sorting (FACS).
Legal Information
1 of 1
This Item | |||
|---|---|---|---|
| description - | description - | description - | description - |
| promoter Promoter name: CMV | promoter Promoter name: CMV | promoter Promoter name: CMV | promoter Promoter name: CMV |
| reporter gene GFP | reporter gene RFP | reporter gene GFP | reporter gene RFP |
| form liquid | form - | form - | form - |
| recombinant expressed in E. coli | recombinant expressed in E. coli | recombinant expressed in E. coli | recombinant expressed in E. coli |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
Storage Class
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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