If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf
CASP3C
Caspase 3 Assay Kit, Colorimetric
Synonym(s):
Colorimetric Caspase-3 Activity Assay Kit
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 1 kit | Check Cart for Availability | $1,120.00 |
About This Item
Quality Level
usage
sufficient for 1,000 multiwell tests (in 96-well multiwell plates), sufficient for 100 standard tests (1 ml)
shipped in
dry ice
storage temp.
−20°C
Gene Information
human ... CASP3(836)
General description
The Caspase 3 Colorimetric Assay Kit is based on the hydrolysis of acetyl-Asp-Glu-Val-Asp p-nitroanilide (Ac-DEVD-pNA) by caspase 3, resulting in the release of the p-nitroaniline (pNA) moiety. p-Nitroaniline is detected at 405 nm (εmM=10.5). The concentration of the pNA released from the substrate is calculated from either the absorbance values at 405 nm or from a calibration curve prepared with pNA standards (pNA standard included with the kit).
Application
Other Notes
1 of 1
This Item | |||
|---|---|---|---|
| Gene Information human ... CASP3(836) | Gene Information human ... CASP3(836) | Gene Information human ... CASP3(836) | Gene Information human ... CASP3(836) |
| Quality Level 300 | Quality Level 200 | Quality Level 100 | Quality Level 100 |
| usage sufficient for 1,000 multiwell tests (in 96-well multiwell plates), sufficient for 100 standard tests (1 ml) | usage sufficient for 1,000 multiwell tests, sufficient for 100 standard tests (2 ml) | usage - | usage - |
| storage temp. −20°C | storage temp. −20°C | storage temp. - | storage temp. - |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
Kit Components Only
- Water (17-18 megaOhm) 125 mL
signalword
Danger
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 3 - STOT RE 2
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
flash_point_f
415.4 °F - closed cup
flash_point_c
213 °C - closed cup
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Articles
Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.
Global Trade Item Number
| SKU | GTIN |
|---|---|
| CASP3C-1KT | 04061835521760 |
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How can I determine the shelf life / expiration / retest date of this product?
1 answer-
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Hola, Me gustaría saber si este kit sirve para detectar activación de caspasa 3 en células adherentes de insulinoma de ratón. En general en células adherentes de ratón. gracias
1 answer-
No data is available regarding the use of this kit with adherent cells; the kit was only tested using Jurkat cells in suspension. For possible usage with adherent cells, it is suggested to consult the literature. Since detachment of adhered cells might induce spontaneous apoptosis, determining the correct conditions for experimental cell induction and detachment before the assay is necessary.
Helpful?
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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