Documents

BICR 78

04072111

Pricing and availability is not currently available.

biological source

human mouth

growth mode

Adherent

karyotype

Aneuploid

morphology

Epithelial, keratinocyte

products

Not specified

receptors

Epidermal Growth Factor Receptor (EGFR)

application(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Related Categories

Cell Line Origin

Oral alveolus squamous carcinoma

Cell Line Description

Adherent keratinocyte cell line derived from a squamous cell carcinoma of the oral alveolus of a Caucasian male. Keratin and involucrin markers present. Known mutations: p53, p16 and p14ARF. Cells are not tumourigenic in athymic mice.

Application

Study of genotype/phenotype relationships

Culture Medium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 0.4 micrograms/ml Hydrocortisone.

Subculture Routine

Split sub-confluent cultures (70-80%) 1:10 - 1:50 using 0.25% trypsin/EDTA; 8% CO2; 37 °C. Suggested seeding density 2 x 10,000 cells/cm2.

Culture cells on a feeder layer of lethally-irradiated or mitomycin C-treated 3T3 Swiss Albino cells (Sigma catalog number 85022108). Feeder layers are prepared in the flasks at least 24 h in advance of being required. Where frozen stocks of treated 3T3 Swiss Albino cells have been prepared, an ampoule is thawed in 37 °C water bath and the contents quickly transferred to a 15 ml centrifuge tube. DMEM medium is added drop wise to 5 ml. Cells are centrifuged at 150 x g for 5 min at room temperature. Cells are resuspended in 5 ml of medium. Cells are counted and added to flasks containing the correct BICR growth medium at 1-3 x 104 cells/cm2.

Other Notes

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Disclaimer

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.

RIDADR

NONH for all modes of transport

Certificate of Analysis
Certificate of Origin

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