Cell Line Origin
Oral alveolus squamous carcinoma
Cell Line Description
Adherent keratinocyte cell line derived from a squamous cell carcinoma of the oral alveolus of a Caucasian male. Keratin and involucrin markers present. Known mutations: p53, p16 and p14ARF. Cells are not tumourigenic in athymic mice.
Study of genotype/phenotype relationships
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 0.4 micrograms/ml Hydrocortisone.
Split sub-confluent cultures (70-80%) 1:10 - 1:50 using 0.25% trypsin/EDTA; 8% CO2; 37 °C. Suggested seeding density 2 x 10,000 cells/cm2.
Culture cells on a feeder layer of lethally-irradiated or mitomycin C-treated 3T3 Swiss Albino cells (Sigma catalog number 85022108). Feeder layers are prepared in the flasks at least 24 h in advance of being required. Where frozen stocks of treated 3T3 Swiss Albino cells have been prepared, an ampoule is thawed in 37 °C water bath and the contents quickly transferred to a 15 ml centrifuge tube. DMEM medium is added drop wise to 5 ml. Cells are centrifuged at 150 x g for 5 min at room temperature. Cells are resuspended in 5 ml of medium. Cells are counted and added to flasks containing the correct BICR growth medium at 1-3 x 104 cells/cm2.
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This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.