MRC-5 PD 19 Cell Line human

05072101

biological source

human lung

description

Human fetal lung

growth mode

Adherent

karyotype

2n=46, diploid

morphology

Fibroblast-like

products

Not specified

receptors

Not specified

application(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

Related Categories

Cell Line Origin

Human foetal lung

Cell Line Description

Established from normal lung tissue of a 14 week old male foetus. The cells undergo between 60-70 population doublings before senescence. The virus susceptibility of this line to human viruses is similar to WI-38.

Application

It has been used as a cell substrate to study the production of recombinant MVA (modified vaccinia virus Ankara).

DNA Profile

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 11,14
D16S539: 9,11
D5S818: 11,12
D7S820: 10,11
THO1: 8
TPOX: 8
vWA: 15

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum FBS / FCS.

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% Trypsin or Trypsin/EDTA; 5% CO2; 37°C.

Other Notes

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Certificate of Analysis

Certificate of Origin

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