Cell Line Origin
Human foetal lung
Cell Line Description
Established from normal lung tissue of a 14 week old male foetus. The cells undergo between 60-70 population doublings before senescence. The virus susceptibility of this line to human viruses is similar to WI-38.
It has been used as a cell substrate to study the production of recombinant MVA (modified vaccinia virus Ankara).
STR-PCR Data: Amelogenin: X,Y
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% Trypsin or Trypsin/EDTA; 5% CO2; 37°C.
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