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06072606, from human bone marrow

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biological source

human bone marrow

growth mode



Hyperdiploid with complex aberrant chromosomes


Round to polygonal cells growing in clusters in suspension


Not specified


Not specified


cell culture | mammalian: suitable

relevant disease(s)


shipped in

dry ice

Related Categories

Cell Line Origin

Human multiple myeloma

Cell Line Description

Established in 1984 from the bone marrow of a 67-year old woman diagnosed with myeloma who survived for one year after diagnosis.

DNA Profile

Not specified

Culture Medium

RPMI 1640 + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS)

Subculture Routine

Doubling time approximately 30 hours. Cells grow singly in suspension. Maintain cultures between 5-20 x 105 cells/ml; 5% CO2; 37 °C. Thaw ampoule and perform a cell count, spin out the DMSO and resuspend in an appropriate volume of media to give a cell concentration of 5 x 105 cells/ml. At subculture perform a cell count and maintain cultures between 5-20 x 105 cells/ml, this tends to equate to a 1:3 split every 2-3 days.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.


This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.

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