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KARPAS-929

06072606, from human bone marrow

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Synonym(s):
KARPAS 929, KARPAS, 929, KARPAS929

biological source

human bone marrow

growth mode

Suspension

karyotype

Hyperdiploid with complex aberrant chromosomes

morphology

Round to polygonal cells growing in clusters in suspension

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

Related Categories

Cell Line Origin

Human multiple myeloma

Cell Line Description

Established in 1984 from the bone marrow of a 67-year old woman diagnosed with myeloma who survived for one year after diagnosis.

DNA Profile

Not specified

Culture Medium

RPMI 1640 + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS)

Subculture Routine

Doubling time approximately 30 hours. Cells grow singly in suspension. Maintain cultures between 5-20 x 105 cells/ml; 5% CO2; 37 °C. Thaw ampoule and perform a cell count, spin out the DMSO and resuspend in an appropriate volume of media to give a cell concentration of 5 x 105 cells/ml. At subculture perform a cell count and maintain cultures between 5-20 x 105 cells/ml, this tends to equate to a 1:3 split every 2-3 days.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Disclaimer

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.

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