Documents

CAD cell line from mouse

08100805

biological source

mouse nerve

description

Mouse (B6/D2 F1 hybrid) catecholaminergic neuronal tumor

growth mode

Adherent

karyotype

Not specified

morphology

Neuronal

products

Not specified

receptors

Not specified

application(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Related Categories

Cell Line Origin

Mouse (B6/D2 F1 hybrid) catecholaminergic neuronal tumour

Cell Line Description

CAD cells (Cath.-a-differentiated) are a variant of a CNS catecholaminergic cell line established from a brain tumour in a transgenic mouse carrying wild-type SV40 T antigen under transcriptional control of rat tyrosine hydroxylase promoter. CAD has lost the immortalising oncogene and expresses neurone-specific proteins and synaptic vesicle proteins. CAD will differentiate to form neuronal processes in serum free conditions.

Application

Study of neuronal differentiation

Culture Medium

DMEM:HAMS F12 (1:1) + 2mM Glutamine + 8% Foetal Bovine Serum (FBS)

Subculture Routine

Split sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at 3 X 104 cells/cm2 Cells should be dislodged by gentle pipetting; 5% CO2; 37°C.

Other Notes

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WGK Germany

3

Certificate of Analysis
Certificate of Origin

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