Cell Line Origin
Mouse malignant mesothelioma
Cell Line Description
Mice (CBA strain female 6-8 weeks) were exposed to crocidiolite asbestos through intraperitoneal injection resulting in tumour development (both ascites and solid tumours). Cultures were established from malignant mesothelial cells obtained from ascites fluid. Cells are tumourigenic in syngeneic imunocompetent mice. Cells grow as adherent monolayers and are epithelial like. Cell Bank Australia reference number: CBA0152
Investigation of the properties of malignant mesothelioma cells and the development of new diagnostic methods.
RPMI1640 (with 2mM L-Glutamine+ 25mM HEPES) + 5% FCS
Optimal split ratio 1:8 - 1:16 (seeding density 0.6 x104 cells/cm2).Cells incubated at 37°C with 5% CO2.Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 min.
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Depositor and originator: Richard Lake - University of Western Australia, Australia