Cell Line Origin
Human malignant mesothelioma
Cell Line Description
This cell line was derived from the pleural fluid of a patient with malignant mesothelioma. The patient had known exposure to crocidolite asbestos. JU77 is not tumourigenic in nude mice. Cells express cytokeratin and epithelial membrane antigen (EMA) but not mucin. Cells are spindle shaped with few vacuoles. Cell Bank Australia reference number: CBA0140
Investigation of the properties of malignant mesothelioma cells and the development of new diagnostic methods.
RPMI 1640 (with 2mM L-Glutamine+ 25mM HEPES) + 5% FCS
Optimal split ratio 1:8 (seeding density 0.8 x104 cells/cm2).Cells incubated at 37°C with 5% CO2.Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 min.
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Depositor and originator: Richard Lake - University of Western Australia, Australia