ONE58 cell line human

10092313

Properties

• biological source: human esophagus
• description: Human malignant mesothelioma
• growth mode: Adherent
• karyotype: Not specified
• morphology: Epithelial-like: cells are spindle-shaped with few vacuoles.
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human malignant mesothelioma

Cell Line Description

This cell line was derived from the pleural fluid of a patient with malignant mesothelioma. The patient had known exposure to crocidolite asbestos. ONE58 is tumourigenic in nude mice. Cells express cytokeratin and epithelial membrane antigen (EMA) but not mucin. Cells are spindle shaped with few vacuoles. Cell Bank Australia reference number: CBA0143

Application

Investigation of the properties of malignant mesothelioma cells and the development of new diagnostic methods.

Culture Medium

RPMI 1640 (with 2mM L-Glutamine+25mM HEPES) + 5% FCS

Subculture Routine

Optimal split ratio 1:4 (seeding density 1.2 x 104 cells/cm²).Cells incubated at 37°C with 5% CO₂.Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 min.

Other Notes

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Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Depositor and originator: Richard Lake - University of Western Australia, Australia