MM253

11072804

• Synonym(s): MM 253

Properties

• biological source: human skin
• growth mode: Adherent
• karyotype: Not specified
• morphology: Epithelial
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): metastasis
• shipped in: dry ice

Description

Cell Line Origin

Human melanoma metastatic site lymph node

Cell Line Description

MM253 is a human melanoma derived from a metastatic site (lymph node). Cell Bank Australia reference number CBA-1347

Culture Medium

RPMI 1640 (R0883) (with 2 mM L-L-Glutamine (G7513) + 25 mM HEPES (H0887) + 10% FBS / FCS (F2442).

Subculture Routine

Optimal split ratio 1:4 - 1:8 (seeding density 0.8 x 10⁴ cells/cm²). Cells incubated at 37 °C with 5% CO₂. Harvest the cells using 0.05% Trypsin/EDTA at 37 °C for 5 min.

Other Notes

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Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.