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MM253

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Synonym(s):
MM 253

biological source

human skin

growth mode

Adherent

karyotype

Not specified

morphology

Epithelial

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

metastasis

shipped in

dry ice

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Cell Line Origin

Human melanoma metastatic site lymph node

Cell Line Description

MM253 is a human melanoma derived from a metastatic site (lymph node). Cell Bank Australia reference number CBA-1347

Culture Medium

RPMI 1640 (R0883) (with 2 mM L-L-Glutamine (G7513) + 25 mM HEPES (H0887) + 10% FBS / FCS (F2442).

Subculture Routine

Optimal split ratio 1:4 - 1:8 (seeding density 0.8 x 104 cells/cm2). Cells incubated at 37 °C with 5% CO2. Harvest the cells using 0.05% Trypsin/EDTA at 37 °C for 5 min.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments.  Please check with your local Customer Service representative for more information.
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

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