Cell Line Description
Mouse embryonic fibroblasts (MEFs) derived from ULK1(+/-) ULK2(+/-) knockout mice. MEFs that were isolated and cultured were immortalised by a standard serial passaging protocol. This cell line can be used as a wild-type control with ULK1 knockout mouse embryonic fibroblasts to study ULK1-dependent processes.
DMEM + 10% FCS + 2mM Glutamine
Trypsinize with 0.05% Trypsin/EDTA and inoculate new cultures at 2 x 104 per cm2. Cultures must be incubated in a humidified 5% CO2/95% air incubator at 37oC.
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Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.