Cell Line Origin
Human foetal lung
Cell Line Description
Established from foetal lung tissue. The cells have a finite lifespan and a similar virus susceptibility to the line MRC-5.
Virus studies: rhinoviruses
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
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