MilliporeSigma
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MRC-7

85020203

biological source

human lung (fetal)

growth mode

Adherent

karyotype

2n = 46, diploid

morphology

Fibroblast

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

Related Categories

Cell Line Origin

Human foetal lung

Cell Line Description

Established from foetal lung tissue. The cells have a finite lifespan and a similar virus susceptibility to the line MRC-5.

Application

Virus studies: rhinoviruses

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Certificate of Analysis

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Certificate of Origin

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