MRC-7

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC Code: 41106514
• Biological source: human lung (fetal)
• Growth mode: Adherent
• Karyotype: 2n = 46, diploid
• Morphology: Fibroblast

Properties

• Product Name: MRC-7, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
• biological source: human lung (fetal)
• packaging: tube of 5 μg 85020203-DNA-5UG; pkg of vial of cells 85020203-1VL
• growth mode: Adherent
• karyotype: 2n = 46, diploid
• morphology: Fibroblast
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Description

Application

Virus studies: rhinoviruses

Biochem/physiol Actions

Established from foetal lung tissue. The cells have a finite lifespan and a similar virus susceptibility to the line MRC-5.

Human foetal lung

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Preparation Note

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.