Documents

CHO Cell Line from Chinese hamster ovary

from hamster ovary, 85050302

Synonym(s):
Chinese hamster ovary cell line
Pricing and availability is not currently available.

biological source

hamster ovary

availability

not available in Japan

growth mode

Adherent

karyotype

Hypodiploid, modal no. 20

morphology

Epithelial

products

Not specified

receptors

Not specified

application(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

Related Categories

Cell Line Origin

Hamster Chinese ovary

Cell Line Description

A subclone of the parental CHO cell line originated by Puck in 1957. The cells have an absolute requirement for L-proline.

Application

Nutritional and gene expression studies
CHO Cell Line from Chinese hamster ovary has been used:
  • to generate CHO cells expressing LY6/PLAUR domain containing 3 (LYPD3)
  • to carry out the vaginolysin (VLY) cytolytic activity assay
  • as host cells to produce glucagon-like peptide 1 receptor (GLP1R)

Culture Medium

Ham′s F12 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

RIDADR

NONH for all modes of transport

WGK Germany

3

Certificate of Analysis
Certificate of Origin
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