This product is a cell line supplied by ECACC. The supplier notes that all cell lines are handled at containment level 2 in accordance with the Advisory Committee on Dangerous Pathogens (ACDP). This cell line was established in 1958, see J Exp Med 1958;108:945. For additional information please see the link below to review the supplier information:
https://www.culturecollections.org.uk/nop/product/cho
CHO Cell Line from Chinese hamster ovary
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 85050302, hamster ovary, Epithelial
Synonym(s):
CHO Cells, CHO-ori Cells, Chinese Hamster Ovary Cells, Chinese hamster ovary cell line
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About This Item
UNSPSC Code:
41106514
Pricing and availability is not currently available.
Recommended Products
biological source
hamster ovary
packaging
tube of 5 μg 85050302-DNA-5UG
pkg of vial of cells 85050302-1VL
availability
not available in Japan
growth mode
Adherent
karyotype
Hypodiploid, modal no. 20
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Hamster Chinese ovary
Cell Line Description
A subclone of the parental CHO cell line originated by Puck in 1957. The cells have an absolute requirement for L-proline.
Application
CHO Cell Line from Chinese hamster ovary has been used:
Nutritional and gene expression studies
Culture Medium
Ham′s F12 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
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Hi there, CHO cells can be used at BSL I lab, right? Best regards, Cristina
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