Documents

MIA-Pa-Ca-2

85062806

biological source

human pancreas

growth mode

Adherent

karyotype

Modal no. 61; hypotriploid

morphology

Epithelial

products

Not specified

receptors

Not specified

application(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Related Categories

Cell Line Origin

Human Caucasian pancreatic carcinoma

Cell Line Description

An established cell line from an undifferentiated human pancreatic carcinoma. The tumour was taken from a 65 year old Caucasian male. The cells are large with abundant cytoplasm, exhibit a high degree of aneuploidy, have a tendency to grow on top of other cells, eventually growing free in suspension. Sensitive to L-Asparaginase.

Application

MIA-Pa-Ca-2 has been used for the assessment of thyroid receptor transcriptional activity and for α-enolase immunostaining.

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 12,13
D16S539: 10,13
D5S818: 12,13
D7S820: 12,13
THO1: 9,10
TPOX: 9
vWA: 15

Culture Medium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

RIDADR

NONH for all modes of transport

Certificate of Analysis
Certificate of Origin
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We offer more than twenty ECACC pancreatic cancer cell lines of human, rat, or mouse origin, including PANC-1. Choose cells based on mutations in KRAS, TP53, SMAD4, and other key genes, or by demonstrated suitability for applications such as toxicity, xenograft models, and drug response studies.
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