T47D Cell Line human

85102201

• Synonym(s): T-47-D Cells, T-47D Cells, T47D:A Cells

Properties

• biological source: human breast
• description: Human breast tumor
• growth mode: Adherent
• karyotype: 2n = 46, hypertriploid, modal no. 65
• morphology: Epithelial
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human breast tumour

Cell Line Description

Established from the pleural effusion of a ductal carcinoma of the breast of a 54-year-old female. The cells carry receptors for a variety of steroids.

Application

T47D cell line human has been used:

• to study the effects of a polysaccharide–protein complex (PPC) isolated from metabolites of gut bacteria Raoultella ornithinolytica from the earthworm Dendrobaena veneta against Candida albicans
• has been cultured and incubated with bone marrow-derived macrophages to study their migration and podosome formation
• to study the anti-proliferative effects of two novel classes of potential antimetabolites on T47D cells

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 11,13
D13S317: 12
D16S539: 10
D5S818: 12
D7S820: 11
THO1: 6
TPOX: 11
vWA: 14

Culture Medium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

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