Documents

293 Cell Line human

from human kidney(embryonic), 85120602

biological source

human kidney (embryonic)

growth mode

Adherent

karyotype

2n 46, hypotriploid, modal no. 64

morphology

Epithelial

products

Not specified

receptors

Not specified

application(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

Related Categories

Cell Line Origin

Human Embryo Kidney

Cell Line Description

Transformed with sheared human Ad5 DNA. Sensitive to human adenoviruses and adenovirus DNA. Can be used to isolate transformation defective host-range mutants of Ad5 and for titrating human adenoviruses. This is a hypotriploid human cell line. The modal chromosome number was 64, occurring in 30% of cells. The rate of cells with higher ploidies was 4.2%. The der (1)t(1;15) (q42;q13), der(19)t(3;19)(q12;q13),der(12)t(8;12) (q22;p13) and four other marker chromosomes were common to most cells. Five other markers occurred in some cells only. The marker der(1) and M8 (or Xq+) were often paired. There were four copies of N17 and N22. Noticeably in addition to three copies of X chromosomes, there were paired Xq+ and a single Xp+ in most cells. The Ad insert was shown to consist of a colinear segment from nucleotides 1 to 4344 integrated into chromosome 19 (19q13.2). Expression of an unusual cell surface receptor for vitronectin has been reported. This is composed of the integrin β-1 subunit and the vitronectin receptor α-v subunit. Detach at room temperature; may take up to seven days to reattach.

Application

Transformation studies
The human embryonic kidney 293 cell line has been used:
  • to study the effects of the Bt insecticidal toxins Cry1Ab and Cry1Ac alone, or with a glyphosate-based herbicide.
  • for toxicological studies of ethoxylated adjuvants of glyphosate-based herbicides.
  • for stable expression of either type 1 or type 2 11-βHSD (11 β-hydroxysteroid dehydrogenase) after transfection with 11-βHSD cDNA.
  • as a host to transfect TLR4 cDNA (HEK-TLR4) to express TLR4 mRNA and protein and induce IL-8 (interleukin-8) promoter activity in response to NE (neutrophil elastase).

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 12,14
D16S539: 9,13
D5S818: 8,9
D7S820: 11,12
THO1: 7,9.3
TPOX: 11
vWA: 16,19

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% FCS

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. When resuscitating a frozen ampoule of cells we recommend the cells are seeded at the higher seeding level i.e. 5 x10,000/cm². Cells may take up to 7 days to attach after resuscitation and subculture. Cells detach easily at room temperature or during transit, therefore growing cultures may be received with cells in suspension. In this event, centrifuge contents of flask and reseed to allow re-attachment of cells. These cells should be frozen in 5% DMSO : 95% FCS.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

RIDADR

NONH for all modes of transport

WGK Germany

3

Certificate of Analysis
Certificate of Origin
  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

  5. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Articles
Eukaryotic cell lines aid in complex post-translational modifications and folding of recombinant proteins. Find suitable recombinant protein production cell lines at sigmaaldrich.com
Read More
DNA, RNA, cDNA derived from ECACC mammalian cell lines allow screening for genes or expression patterns to identify lines most suitable for specific research.
Read More

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

MilliporeSigma

Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.