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MilliporeSigma

MCF7 Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 86012803, human breast (adenocarcinoma), Epithelial-like

Synonym(s):

IBMF-7 Cells, MCF7 Cells, Michigan Cancer Foundation-7 Cells

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About This Item

UNSPSC Code:
41106514
eCl@ss:
32190102

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biological source

human breast (adenocarcinoma)

packaging

tube of 5 μg 86012803-DNA-5UG
pkg of vial of cells 86012803-1VL

growth mode

Adherent

karyotype

2n = 46, hypertriploid to hypotetraploid

morphology

Epithelial-like

products

Not specified

receptors

Reported to express the oestrogen and progesterone receptor

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Related Categories

Application

Tumourigenicity and virus studies: Type B and C viruses

Biochem/physiol Actions

Established from the pleural effusion from a 69 year female caucasian suffering from a breast adenocarcinoma. Cells exhibit some features of differentiated mammary epithelium including oestradiol synthesis and formation of domes. Cells may carry B or C type retrovirus and are considered to represent a category 2 pathogen (P2 containment). Cells express both the wild type and variant oestrogen receptors as well as progesterone receptor. The cells should be handled under laboratory containment level 2.
Human Caucasian breast adenocarcinoma
STR-PCR Data:
Amelogenin: X
CSF1PO: 10
D13S317: 11
D16S539: 11,12
D5S818: 11,12
D7S820: 8,9
THO1: 6
TPOX: 9,12
vWA: 14,15

Preparation Note

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids NEAA + 10% Foetal Bovine Serum FBS / FCS.
Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% Trypsin or Trypsin/EDTA; 5% CO2; 37°C.

Other Notes

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Questions

1–8 of 8 Questions  

  1. Does this grow on RPMI? If so, what additional supplements does it need? If not, what recommended medium to be used to culture these cells?

    1 answer

    1. Product 86012803, MCF7 Cell Line human, is not tested with RPMI. The recommended Culture Medium is EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids NEAA + 10% Foetal Bovine Serum FBS / FCS.

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  2. Are the Cell Line cells tested for viral pathogens?

    1 answer

    1. HPA Cultures does not perform any viral testing on the cell lines.

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  3. Why is Vapor Phase Liquid Nitrogen preferred for storage of Cell Line cells?

    1 answer

    1. If ampules are immersed into liquid phase of liquid nitrogen, it increases the risk of the liquid seeping into the vial. This could lead to problems of cross-contamination and increased risk of the ampule exploding when thawed.

      Helpful?

  4. What medium should I use when I thaw the Cell Line cells?

    1 answer

    1. The medium for each cell line is listed on the product page. It is specific to each cell line. If not visible on the Sigma-Aldrich website product page, the HPA website also contains the same information (www.hpacultures.org.uk). The product number for the cell line is the same on either site.

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  5. Are my Cell Line cells mycoplasma tested?

    1 answer

    1. ECACC routinely tests all manufactured cell banks for mycoplasma.

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  6. How many Cell Line cells are in the vial?

    1 answer

    1. Each vial of cells contains 2-3 x 106 cells in 1 ml of freezing media. This is in a 1.8 mL ampule.  For exceptionally large cells, counts may decrease. Suspension cells, generally smaller cells, may contain as many as 4-5 x 106 cells/vial to assure optimal viability upon thaw.

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  7. How do I handle Cell Line frozen cells upon arrival?

    1 answer

    1. Upon receipt, frozen ampules should be transferred directly to vapor phase liquid nitrogen without delay (-135°C) or liquid phase liquid nitrogen if vapor phase is not available. DO NOT use a -80°C freezer as an alternative; this will result in loss of viability.

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  8. What passage are my Cell Line cells?

    1 answer

    1. If the passage of the cells is known, it is listed on the product page at the HPA website (www.hpacultures.org.uk).

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