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Hep2 (HeLa derivative) Cell Line human

86030501, human cervix, Epithelial

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About This Item

UNSPSC Code:
41106514

product name

Hep2 (HeLa derivative) Cell Line human, 86030501

biological source

human cervix

description

Human Black, cervix carcinoma

growth mode

Adherent

karyotype

Modal no. 14

morphology

Epithelial

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Human cervix carcinoma

Cell Line Description

Originated from tumours produced in irradiated-cortisonised weanling rats after injecting with epidermoid carcinoma tissue from the larynx of a 56 year old male. This cell line was found to be indistinguishable from HeLa by STR PCR DNA profiling. Therefore, the cell line should be considered as derived from HeLa. Ethnicity: Black.

Application

Human Hep2 (HeLa derivative) cell line has been used for virus and tumorigenicity studies.

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. After thawing the first subculture may take 7-10 days.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

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