Cell Line Origin
Mouse C57BL/1CRF lung carcinoma
Cell Line Description
Derived from an alveologenic lung carcinoma from a C57BL/1CRF mouse. Can be maintained in a completely defined serum free medium, but the growth rate is much slower than in supplemented serum. Used in studies of lung metastases.
Waymouth′s MB 752/1 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
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This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.