COLO 201

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 87091201, human colon. Clumps of spherical cells in suspension, cuboidal cells in monolayer.

• UNSPSC Code: 41106514
• Biological source: human colon
• Relevant disease(s): cancer
• Growth mode: Adherent
• Karyotype: Triploid modal no. 71-78
• Morphology: Clumps of spherical cells in suspension, cuboidal cells in the monolayer

Properties

• biological source: human colon
• packaging: tube of 5 μg 87091201-DNA-5UG, pkg of vial of cells 87091201-1VL
• growth mode: Adherent
• karyotype: Triploid modal no. 71-78
• morphology: Clumps of spherical cells in suspension, cuboidal cells in the monolayer
• products: Carcinoembryonic antigen (CEA) less than produced by COLO 205
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Biochem/physiol Actions

COLO 201, COLO 205 and COLO 206 (ECACC Catalogue 93052620) were all established from the ascites fluid obtained from a 70-year-old male patient with adenocarcinoma of the colon (Dukes Classification Grade D). At time of isolation, COLO 201 displayed cuboidal morphology but with increasing passage the depositor reported that COLO 201 tends to display predominantly rounded cells in clumps in suspension with cuboidal cells in the monolayer. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.

Human Caucasian colon adenocarcinoma

STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 10
D16S539: 12,13
D5S818: 10,13
D7S820: 9,10
THO1: 8,9
TPOX: 11
vWA: 15

Preparation Note

Maintain suspension cells at 3-9x10⁵ cells/cm³. Split sub-confluent cultures (70-80%) of attached cells 1:3 to 1:6 i.e. seeding at 2-3x10⁴ cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.