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Jurkat E6.1 Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 88042803, human blood (leukemic T-cell lymphoblast), Not specified

Synonym(s):

Jurkat Cells, Jurkat Clone E6-1 Cells

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Estimated to ship TODAYfromSAINT LOUIS

$436.00
1 vial

Estimated to ship TODAYfromSAINT LOUIS

$610.00

About This Item

UNSPSC Code:
41106514
Biological source:
human blood (leukemic T-cell lymphoblast)
Relevant disease(s):
cancer
Growth mode:
Suspension
Karyotype:
Pseudodiploid, modal No 46
Morphology:
Not specified

$436.00

Estimated to ship TODAYDetails

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biological source

human blood (leukemic T-cell lymphoblast)

packaging

tube of 5 μg 88042803-DNA-5UG, pkg of vial of cells 88042803-1VL

growth mode

Suspension

karyotype

Pseudodiploid, modal No 46

morphology

Not specified

products

Interleukin 2 (IL-2), human alpha interferon

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Application

IL-2 assay and cloning studies
Jurkat E6.1 cell line has been used to:
  • study cell-independent/vector-free delivery of bovine serum albumin-fluorescein conjugate
  • determine the cytotoxicity of the cytolethal distending toxin (CDT) holotoxin and its components
  • study the expression of bisphenol A exposed estrogen receptor-β (ERβ) and estrogen-related-receptor-α (ERRα) in vitro

Biochem/physiol Actions

Derived from Jurkat FHCRC. An IL-2 producing cell line, derived by incubating the cells at 41°C for 48 hours followed by a limiting dilution cloning over macrophages.
Human leukaemic T cell lymphoblast
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 8,12
D16S539: 11
D5S818: 9
D7S820: 8,10
THO1: 6,9.3
TPOX: 8,10
vWA: 18

Preparation Note

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. On resuscitation single cells can be observed, during culture most cells will grow as aggregates.
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to [email protected].

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Protocols

T-Cell Migration Assays Using Millicell® Cell Culture Inserts

Learn how to perform cell migration assays in vitro using Millicell® hanging cell culture inserts and the suspension T-cell lines Jurkat and primary CD4+ cells. Monitor migration by flow cytometry and EZ-MTT assays.

Articles

Dynamic Live Cell Imaging of Caspase Mediated Apoptosis and Cellular Hypoxia in Lymphocytes and Cancer Cells Using the CellASIC® ONIX2 Microfluidic Platform

Application note on how the CellASIC® ONIX2 microfluidic system can be used to analyze caspase-3 mediated apoptosis/cell death and cellular hypoxia in live immune and cancer cell lines.

Questions

1–3 of 3 Questions  

  1. How many viable cells are in the vial of Jurkat E3.1 cells?

    1 answer

    1. This product is a suspension cell line which is packaged as 4-5 million cells per vial.

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  2. Does this particular batch of Jurkat E6.1 express CD28?

    1 answer

    1. The Jurkat E6.1 cell line is a banked product of ECACC and is tested sterility, viability, mycoplama, and identity. It is not tested for any specific expression. The depositor listed in Prof H Harris/Dr C Madin, Sir William Dunn School of Pathology, Oxford. The line has not been further modified. Various publications suggest that the E6.1 does express CD28, however we cannot validate these claims. It may be helpful to refer to the literature for further information.

      Helpful?

  3. I am interested in Jurkat cells; product number 88042803 appears to be a vial of these cells for culturing. But why is the extension '-DNA-5UG' in the product number? Does this mean that it is not cells but 5 µg DNA isolated from Jurkat cells???

    1 answer

    1. To order the actual cells, order the SKU with the -1VL suffix, 88042803-1VL. This SKU is for the Jurkat E6.1 cells that can be propagated and maintained in culture. The other SKUs listed there are preparations of DNA, RNA and cDNA from this cell line.

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