SAOS-2 Cell Line human

89050205, primary osteogenic sarcoma

• Synonym(s): SAOS2 Cells

Properties

• biological source: human bone
• growth mode: Adherent
• karyotype: 2n = 46, (P1) Hyperploid to hypopentaploid
• morphology: Epithelial-like
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human primary osteogenic sarcoma

Cell Line Description

Reported to have been derived from an 11 year old female Caucasian. The patient was treated with RTG, methotrexate, adriamycin, vincristine, cytoxan, and aramycin-C. HLA cell line phenotype: A2,3;Bw16,w47.

Application

SAOS-2 cell line has been used to study the importance of cyclin D1 for the activity of lithocholic acid hydroxyamide (LCAHA). It has also been used to study the effect of chromium-enriched diamond-like carbon (DLC) layers on cell adhesion and proliferation.

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 12,13
D16S539: 12,13
D5S818: 12
D7S820: 8,10
THO1: 6,9
TPOX: 8
vWA: 18

Culture Medium

McCoy′s 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.