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K562 Cell line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 89121407, human blood (chronic myelogenous leukemia), Lymphoblast

Synonym(s):

K-562 Cells

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$610.00
1 vial
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$610.00

About This Item

UNSPSC Code:
41106514
Biological source:
human blood (chronic myelogenous leukemia)
Relevant disease(s):
cancer
Growth mode:
Suspension
Karyotype:
2n = 46
Morphology:
Lymphoblast

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biological source

human blood (chronic myelogenous leukemia)

packaging

tube of 5 μg 89121407-DNA-5UG, pkg of vial of cells 89121407-1VL

growth mode

Suspension

karyotype

2n = 46

morphology

Lymphoblast

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Application

Natural killer assay and tumourigenicity studies
K562 cell line has been used to study the protecting role of cluster of differentiation-39 (CD39) attenuation against DNA damage. It has also been used to study the relevance of a novel sorting approach for separating cells, called “Slice and Dice Sorting”.

Biochem/physiol Actions

Established from pleural effusion of 53 year old female with chronic myelogenous leukaemia in terminal blast crisis. Population highly undifferentiated and of the granulocytic series. Can be used as a highly sensitive target for the in vitro natural killer assay. Recent studies have shown the K562 blasts are multipotential, hematopoietic malignant cells that spontaneously differentiate into recognisable progenitors of the erythrocyte, granulocyte and monocytic series.
Human Caucasian chronic myelogenous leukaemia
STR-PCR Data: Amelogenin: X
CSF1PO: 9,10
D13S317: 8
D16S539: 11,12
D5S818: 11,12
D7S820: 9,11
THO1: 9.3
TPOX: 8,9
vWA: 16

Preparation Note

Maintain cultures between 100,000 to 1,000,000 cells/ml; 5% CO2; 37°C.
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.

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Questions

1–9 of 9 Questions  

  1. Do K562 cells express CD34?

    1 answer

    1. The K562 cell line isn't tested for CD markers. This cell line is a product of ECACC and were originally obtained from ATCC. According to various publications these cells do express CD34. See the link below for one of these publications:
      https://plos.figshare.com/articles/figure/Flow_cytometry_of_cell_surface_markers_of_K562_and_K562-IR_cells_/11909724/1

      See the link below for the ECACC product page:
      https://www.culturecollections.org.uk/products/celllines/generalcell/detail.jsp?refId=89121407&collection=ecacc_gc#medDoc

      Helpful?

  2. Is the BCR/ABL cell gene positive?

    1 answer

    1. This cell line is a banked product of ECACC. As stated on the ECACC product page, the line was acquired from ATCC. The original depositor listed at ATCC is HT Holden and appears to have been established sometime in the 1970s. There is no confirmation of the Bcr-Abl by either supplier. However, see the link below for a reference that appears to suggest that this line is Bcr-Abl positive. This publication has not been validated.
      https://pubmed.ncbi.nlm.nih.gov/16465215/

      For more technical information, please visit the website with this product page: https://www.culturecollections.org.uk/products/celllines/generalcell/detail.jsp?refId=89121407&collection=ecacc_gc

      Helpful?

  3. Are the Cell Line cells tested for viral pathogens?

    1 answer

    1. HPA Cultures does not perform any viral testing on the cell lines.

      Helpful?

  4. Are my Cell Line cells mycoplasma tested?

    1 answer

    1. ECACC routinely tests all manufactured cell banks for mycoplasma.

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  5. How do I handle Cell Line frozen cells upon arrival?

    1 answer

    1. Upon receipt, frozen ampules should be transferred directly to vapor phase liquid nitrogen without delay (-135°C) or liquid phase liquid nitrogen if vapor phase is not available. DO NOT use a -80°C freezer as an alternative; this will result in loss of viability.

      Helpful?

  6. Why is Vapor Phase Liquid Nitrogen preferred for storage of Cell Line cells?

    1 answer

    1. If ampules are immersed into liquid phase of liquid nitrogen, it increases the risk of the liquid seeping into the vial. This could lead to problems of cross-contamination and increased risk of the ampule exploding when thawed.

      Helpful?

  7. What passage are my Cell Line cells?

    1 answer

    1. If the passage of the cells is known, it is listed on the product page at the HPA website (www.hpacultures.org.uk).

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  8. How many Cell Line cells are in the vial?

    1 answer

    1. Each vial of cells contains 2-3 x 106 cells in 1 ml of freezing media. This is in a 1.8 mL ampule.  For exceptionally large cells, counts may decrease. Suspension cells, generally smaller cells, may contain as many as 4-5 x 106 cells/vial to assure optimal viability upon thaw.

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  9. What medium should I use when I thaw the Cell Line cells?

    1 answer

    1. The medium for each cell line is listed on the product page. It is specific to each cell line. If not visible on the Sigma-Aldrich website product page, the HPA website also contains the same information (www.hpacultures.org.uk). The product number for the cell line is the same on either site.

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