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Documents
biological source
mouse lung
growth mode
Adherent
karyotype
Not specified
morphology
Not specified
products
Not specified
receptors
Not specified
application(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Related Categories
Cell Line Origin
Mouse C57BL Lewis lung carcinoma
Cell Line Description
Derived from the lung of a C57BL mouse implanted with a primary Lewis Lung carcinoma. The cells can be used as a model for metastasis and for studying the effects of chemotherapeutic agents.
Application
Tumourigenicity, Metastasis and chemotherapeutic agent studies
Culture Medium
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:4 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using mechanical technique (aspiration); 5% CO2; 37°C. Cells detach as aggregates and are therefore difficult to count.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
RIDADR
NONH for all modes of transport
Certificate of Analysis
Certificate of Origin
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